James H. Eberwine

About

James H. Eberwine, Ph.D., is the Elmer Holmes Bobst Professor of Pharmacology at the University of Pennsylvania School of Medicine. He is a member of multiple institutes within the university, including the Institute of Neurological Sciences, the Abramson Cancer Center, the Center for Bioinformatics, the Institute for Medicine and Engineering, the Genomics Institute, and the Center for Cell Engineering. Dr. Eberwine is also a Co-Director of the PENN Program in Single Cell Biology and the Penn Center for Subcellular Genomics. His research focuses on understanding the molecular basis of neuronal functioning, with an emphasis on neuronal adaptation processes such as tetanic potentiation, glucocorticoid-induced, age-induced, and opiate-induced adaptation. His laboratory employs molecular biology techniques, single-cell genetics, cDNA cloning, in situ hybridization, in situ transcription, mRNA amplification, and expression profiling to analyze gene expression at the single-cell level. Dr. Eberwine's work involves analyzing the mRNA and protein complement of individual cells, as well as mRNA movement and translation within single cells, to generate molecular and bioprocess fingerprints of various cell types and disease states. He has contributed to the understanding of subcellular localization of mRNAs in neurons, demonstrating that multiple mRNAs are localized in neuronal dendrites and providing formal proof of local mRNA translation in dendrites. His research has shown that the…

Research topics

• Biology
• Computational biology
• Cell biology
• Neuroscience
• Molecular biology

Selected publications

• Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration

  Developmental Cell · 2020-02-27 · 95 citations

  articleOpen access
  PublisherOA PDFDOI

• Ultrasensitive Single Extracellular Vesicle Detection Using High Throughput Droplet Digital Enzyme-Linked Immunosorbent Assay

  Nano Letters · 2022-05-19 · 79 citations

  articleOpen access

  Extracellular vesicles (EVs) have attracted enormous attention for their diagnostic and therapeutic potential. However, it has proven challenging to achieve the sensitivity to detect individual nanoscale EVs, the specificity to distinguish EV subpopulations, and a sufficient throughput to study EVs among an enormous background. To address this fundamental challenge, we developed a droplet-based optofluidic platform to quantify specific individual EV subpopulations at high throughput. The key inn…

  PublisherOA PDFDOI

• Micro‐ and Nano‐Devices for Studying Subcellular Biology

  Small · 2020-12-20 · 23 citations

  reviewOpen access

  Abstract Cells are complex machines whose behaviors arise from their internal collection of dynamically interacting organelles, supramolecular complexes, and cytoplasmic chemicals. The current understanding of the nature by which subcellular biology produces cell‐level behaviors is limited by the technological hurdle of measuring the large number (>10 3 ) of small‐sized (<1 μm) heterogeneous organelles and subcellular structures found within each cell. In this review, the emergence of a su…

  PublisherOA PDFDOI

• Subcellular omics: a new frontier pushing the limits of resolution, complexity and throughput

  Nature Methods · 2023-03-01 · 18 citations

  articleOpen access1st authorCorresponding
  PublisherOA PDFDOI

• Astrocytes promote ethanol-induced enhancement of intracellular Ca2+ signals through intercellular communication with neurons

  iScience · 2021-04-19 · 14 citations

  articleOpen access

  Ethanol (EtOH) abuse induces significant mortality and morbidity worldwide because of detrimental effects on brain function. Defining the contribution of astrocytes to this malfunction is imperative to understanding the overall EtOH effects due to their role in homeostasis and EtOH-seeking behaviors. Using a highly controllable in vitro system, we identify chemical signaling mechanisms through which acute EtOH exposure induces a modulatory feedback loop between neurons and astrocytes. Neuronally…

  PublisherOA PDFDOI

Recent grants

• NIH Grant U01MH09895304

  NIH · $2.3M · 2016

• NIH Grant R37AG009900

  NIH · $3.4M · 2011

• NIH Grant R01MH058561

  NIH · $2.8M · 2008

Frequent coauthors

• Junhyong Kim

  California University of Pennsylvania

  57 shared

• Kevin Miyashiro

  Translational Therapeutics (United States)

  42 shared

• Jai‐Yoon Sul

  Translational Therapeutics (United States)

  37 shared

• Jennifer Spaethling

  Translational Therapeutics (United States)

  28 shared

• Stephen Fisher

  26 shared

• Peter B. Crino

  University of Maryland, College Park

  25 shared

• Bernhard Kühn

  Children's Hospital of Pittsburgh

  24 shared

• Balakrishnan Ganapathy

  Biogen (United States)

  22 shared

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