Eric Betzig

About

Eric Betzig is a Howard Hughes Medical Institute Investigator and a Professor of Cell Biology, Development and Physiology at the University of California, Berkeley. His research focuses on molecular and cell biology, with particular emphasis on advanced imaging techniques. He is involved in exploring the frontiers of microscopy and cellular imaging to better understand biological processes at the molecular level.

Research topics

• Biophysics
• Biology
• Biochemistry
• Geometry
• Artificial Intelligence
• Physics
• Computer Science
• Cell biology
• Materials science
• Optics

Selected publications

• Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells

  Science · 2020 · 361 citations

  Within cells, the spatial compartmentalization of thousands of distinct proteins serves a multitude of diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) can elucidate protein spatial relationships to global ultrastructure, but has suffered from tradeoffs of structure preservation, fluorescence retention, resolution, and field of view. We developed a platform for three-dimensional cryogenic SR and focused ion beam-milled block-face EM across en…

  DOI

• Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator

  Neuron · 2023 · 128 citations

  The ability to optically image cellular transmembrane voltages at millisecond-timescale resolutions can offer unprecedented insight into the function of living brains in behaving animals. Here, we present a point mutation that increases the sensitivity of Ace2 opsin-based voltage indicators. We use the mutation to develop Voltron2, an improved chemigeneic voltage indicator that has a 65% higher sensitivity to single APs and 3-fold higher sensitivity to subthreshold potentials than Voltron. Voltr…

  PublisherOA PDFDOI

• Image processing tools for petabyte-scale light sheet microscopy data

  Nature Methods · 2024-10-17 · 39 citations

  articleOpen access

  Light sheet microscopy is a powerful technique for high-speed three-dimensional imaging of subcellular dynamics and large biological specimens. However, it often generates datasets ranging from hundreds of gigabytes to petabytes in size for a single experiment. Conventional computational tools process such images far slower than the time to acquire them and often fail outright due to memory limitations. To address these challenges, we present PetaKit5D, a scalable software solution for efficient…

  PublisherDOI

• Characterization, comparison, and optimization of lattice light sheets

  Science Advances · 2023-03-31 · 33 citations

  articleOpen accessCorresponding

  Lattice light sheet microscopy excels at the noninvasive imaging of three-dimensional (3D) dynamic processes at high spatiotemporal resolution within cells and developing embryos. Recently, several papers have called into question the performance of lattice light sheets relative to the Gaussian sheets most common in light sheet microscopy. Here, we undertake a theoretical and experimental analysis of various forms of light sheet microscopy, which demonstrates and explains why lattice light sheet…

  PublisherOA PDFDOI

• u-track3D: Measuring, navigating, and validating dense particle trajectories in three dimensions

  Cell Reports Methods · 2023-12-01 · 33 citations

  articleOpen access

  We describe u-track3D, a software package that extends the versatile u-track framework established in 2D to address the specific challenges of 3D particle tracking. First, we present the performance of the new package in quantifying a variety of intracellular dynamics imaged by multiple 3D microcopy platforms and on the standard 3D test dataset of the particle tracking challenge. These analyses indicate that u-track3D presents a tracking solution that is competitive to both conventional and deep…

  PublisherDOI

Frequent coauthors

• Srigokul Upadhyayula

  University of California, Berkeley

  150 shared

• Daniel E. Milkie

  Janelia Research Campus

  100 shared

• Tomas Kirchhausen

  Boston Children's Hospital

  99 shared

• Bi‐Chang Chen

  Research Center for Applied Science, Academia Sinica

  95 shared

• Wesley R. Legant

  North Carolina State University

  88 shared

• Jennifer Lippincott‐Schwartz

  Janelia Research Campus

  88 shared

• Tian-Ming Fu

  Princeton University

  75 shared

• Harald F. Hess

  Integral Consulting (United States)

  71 shared

Education

• Ph.D., Physics

  University of California, Berkeley

  1990

• B.S., Physics

  Princeton University

  1984

Similar researchers at University of California, Berkeley

• Carlos Bustamanteh-164
• David G. Drubinh-103
• Andrew Dillinh-100