David G. Drubin

About

David G. Drubin is the Ernette Comby Chair in Microbiology and a Professor of Cell Biology, Development and Physiology at the University of California, Berkeley. His research description can be found at http://mcb.berkeley.edu/faculty/CDB/drubind.html and his laboratory's website is http://drubinbarneslab.berkeley.edu/. He is involved in the field of molecular and cell biology, with a focus on microbiology, cell biology, development, and physiology. Drubin's work contributes to understanding cellular processes and microbiological mechanisms, although specific details of his research focus are not provided in the page text.

Research topics

• Computer Science
• Nanotechnology
• Biophysics
• Artificial Intelligence
• Materials science
• Cell biology
• Biology
• Physics
• Biochemistry
• Optics

Selected publications

• Principles of self-organization and load adaptation by the actin cytoskeleton during clathrin-mediated endocytosis

  eLife · 2020 · 164 citations

  Senior authorCorresponding

  Force generation by actin assembly shapes cellular membranes. An experimentally constrained multiscale model shows that a minimal branched actin network is sufficient to internalize endocytic pits against membrane tension. Around 200 activated Arp2/3 complexes are required for robust internalization. A newly developed molecule-counting method determined that ~200 Arp2/3 complexes assemble at sites of clathrin-mediated endocytosis in human cells. Simulations predict that actin self-organizes into…

  PublisherDOI

• Mechanistic insights into actin force generation during vesicle formation from cryo-electron tomography

  Developmental Cell · 2022 · 49 citations

  Senior authorCorresponding
  PublisherOA PDFDOI

• Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

  Scientific Reports · 2020 · 43 citations

  Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system in…

  DOI

• Endocytic myosin-1 is a force-insensitive, power-generating motor

  The Journal of Cell Biology · 2023-08-07 · 19 citations

  articleOpen accessCorresponding

  Myosins are required for clathrin-mediated endocytosis, but their precise molecular roles in this process are not known. This is, in part, because the biophysical properties of the relevant motors have not been investigated. Myosins have diverse mechanochemical activities, ranging from powerful contractility against mechanical loads to force-sensitive anchoring. To better understand the essential molecular contribution of myosin to endocytosis, we studied the in vitro force-dependent kinetics of…

  PublisherOA PDFDOI

• A role for cross-linking proteins in actin filament network organization and force generation

  Proceedings of the National Academy of Sciences · 2024-10-15 · 6 citations

  articleOpen accessSenior authorCorresponding

  The high turgor pressure across the plasma membrane of yeasts creates a requirement for substantial force production by actin polymerization and myosin motor activity for clathrin-mediated endocytosis (CME). Endocytic internalization is severely impeded in the absence of fimbrin, an actin filament crosslinking protein called Sac6 in budding yeast. Here, we combine live-cell imaging and mathematical modeling to gain insights into the role of actin filament crosslinking proteins in force generatio…

  PublisherOA PDFDOI

Recent grants

• NIH Grant R01GM042759

  NIH · $3.6M · 2006

• Endocytic Functions for the Mammalian Actin Cytoskeleton

  NIH · $3.4M · 2002–2016

• Actin assembly and clathrin-mediated endocytosis in yeast and mammals

  NIH · $10.9M · 2016–2026

Frequent coauthors

• Georjana Barnes

  University of California, Berkeley

  51 shared

• John R. Yates

  Scripps Research Institute

  24 shared

• Yidi Sun

  University of California, Berkeley

  22 shared

• Avital A. Rodal

  Brandeis University

  22 shared

• Pekka Lappalainen

  University of Helsinki

  22 shared

• Johannes Schöneberg

  University of California, San Diego

  20 shared

• David C. Amberg

  SUNY Upstate Medical University

  17 shared

• Bruce L. Goode

  Brandeis University

  17 shared

Similar researchers at University of California, Berkeley

• Carlos Bustamanteh-164
• Eric Betzigh-116
• Andrew Dillinh-100