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Seungeun Oh

Seungeun Oh

· Professor

University of California, San Diego · Cellular and Molecular Medicine

Active 2000–2024

h-index25
Citations4.7k
Papers5723 last 5y
Funding
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About

Welcome to the Oh lab at UCSD! Our research group is part of the Department of Physics and the Department of Cellular And Molecular Medicine at the University of California, San Diego. We are interested in how life controls its physical properties and are currently focusing on single-cell regulation of protein and lipid mass, volume, and density. We develop cutting-edge label-free optical microscopy tools including quantitative phase microscopy and nonlinear optical microscopy to explore these phenomenon in cells, small model organisms, and tissues.

Research topics

  • Genetics
  • Biology
  • Pharmacology
  • Computational biology
  • Cell biology
  • Cancer research
  • Chemistry

Selected publications

  • Enhancer release and retargeting activates disease-susceptibility genes

    Nature · 2021 · 150 citations

    1st authorCorresponding
    • Genetics
    • Biology
    • Computational biology
  • Super-Enhancer Redistribution as a Mechanism of Broad Gene Dysregulation in Repeatedly Drug-Treated Cancer Cells

    Cell Reports · 2020 · 40 citations

    • Cancer research
    • Biology
    • Chemistry

    Cisplatin is an antineoplastic drug administered at suboptimal and intermittent doses to avoid life-threatening effects. Although this regimen shortly improves symptoms in the short term, it also leads to more malignant disease in the long term. We describe a multilayered analysis ranging from chromatin to translation-integrating chromatin immunoprecipitation sequencing (ChIP-seq), global run-on sequencing (GRO-seq), RNA sequencing (RNA-seq), and ribosome profiling-to understand how cisplatin confers (pre)malignant features by using a well-established ovarian cancer model of cisplatin exposure. This approach allows us to segregate the human transcriptome into gene modules representing distinct regulatory principles and to characterize that the most cisplatin-disrupted modules are associated with underlying events of super-enhancer plasticity. These events arise when cancer cells initiate without ultimately ending the program of drug-stimulated death. Using a PageRank-based algorithm, we predict super-enhancer regulator ISL1 as a driver of this plasticity and validate this prediction by using CRISPR/dCas9-KRAB inhibition (CRISPRi) and CRISPR/dCas9-VP64 activation (CRISPRa) tools. Together, we propose that cisplatin reprograms cancer cells when inducing them to undergo near-to-death experiences.

Frequent coauthors

  • Michael G. Rosenfeld

    University of California, San Diego

    39 shared
  • Qi Ma

    Xinjiang Medical University

    29 shared
  • Wenbo Li

    27 shared
  • Kenneth A. Ohgi

    University of California, San Diego

    27 shared
  • Daria Merkurjev

    17 shared
  • Sreejith J. Nair

    Georgetown University Medical Center

    17 shared
  • Xiaoyuan Song

    University of Science and Technology of China

    17 shared
  • Chengyu Liang

    15 shared

Labs

Education

  • Ph.D., Physics

    Massachusetts Institute of Technology

    2010

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