Marla C Craig

Supplementary Table 2 from Bone Marrow Microenvironment Niche Regulates miR-221/222 in Acute Lymphoblastic Leukemia

2023-04-03

<p>Excel sheet showing mean signal intensities and p-values of SUP-B15 ALL cells cultured in media only or co-cultured with HOB. Statistics were done as follows: Data were analyzed by first subtracting the background and then normalizing the signals using a LOWESS filter (Locally-weighted Regression) (2). For two color experiments, the ratio of the two sets of detected signals (log2 transformed, balanced) and p-values of the t-test were calculated; differentially detected signals were thos…

Supplementary Table 1 from Bone Marrow Microenvironment Niche Regulates miR-221/222 in Acute Lymphoblastic Leukemia

2023-04-03

<p>Excel sheet showing mean signal intensities and p-values of SUP-B15 ALL cells cultured in media only or co-cultured with BMSC. Statistics were done as follows: Data were analyzed by first subtracting the background and then normalizing the signals using a LOWESS filter (Locally-weighted Regression) (2). For two color experiments, the ratio of the two sets of detected signals (log2 transformed, balanced) and p-values of the t-test were calculated; differentially detected signals were tho…

Supplementary Figure 2- Constitutive overexpression of p27 increases ALL cell chemoresistance from Bone Marrow Microenvironment Niche Regulates miR-221/222 in Acute Lymphoblastic Leukemia

2023-04-03

<p>S2A, REH leukemic cells have overexpressed p27 protein compared to empty vector control. S2B, an increase in viability was observed in REH cells overexpressing p27 compared to empty vector control.</p>

Supplementary Figure 1- PTEN and p57 have increased abundance in ALL cells influenced by the BMM from Bone Marrow Microenvironment Niche Regulates miR-221/222 in Acute Lymphoblastic Leukemia

2023-04-03

<p>Consistent with p27 two other validated miR-221/222 target genes which have been previously shown to play a role in cellular quiescence and survival, PTEN and CDKN1C (p57), were assessed in leukemic cells under conditions mentioned above. S1A,B, both PTEN and p57 protein abundance was increased in leukemic cells co-cultured with BMSC or HOB compared to leukemic cells grown in media alone.</p>

Supplementary Figure 1- PTEN and p57 have increased abundance in ALL cells influenced by the BMM from Bone Marrow Microenvironment Niche Regulates miR-221/222 in Acute Lymphoblastic Leukemia

2023-04-03

<p>Consistent with p27 two other validated miR-221/222 target genes which have been previously shown to play a role in cellular quiescence and survival, PTEN and CDKN1C (p57), were assessed in leukemic cells under conditions mentioned above. S1A,B, both PTEN and p57 protein abundance was increased in leukemic cells co-cultured with BMSC or HOB compared to leukemic cells grown in media alone.</p>