Krzysztof Krajewski

About

Krzysztof Krajewski is a Professor and Director of the Peptide Synthesis Core at the UNC School of Medicine, Department of Biochemistry and Biophysics. His research focuses on peptide chemistry and synthesis, histone post-translational modifications (PTMs), and the applications of peptides in biochemical assays. He has extensive experience in synthesizing peptides up to 80 residues long, utilizing automated microwave-assisted Fmoc Solid Phase Peptide Synthesis (SPPS), and offers a wide range of chemical modifications including acetylation, methylation, phosphorylation, fluorescent labeling, biotinylation, PEG spacers, incorporation of D-amino acids, unnatural amino acids, and stable isotopes. His core facility supports applications in antibody production, enzyme assays, ligand-binding studies, and quantitative biophysical analyses such as NMR, ITC, and crystallography, ensuring high-quality peptides tailored to diverse research needs.

Research topics

• Genetics
• Biology
• Computational biology
• Computer Science
• Evolutionary biology
• Artificial Intelligence
• Nanotechnology
• Cell biology
• Chemistry
• Botany

Selected publications

• Characterization of the plant homeodomain (PHD) reader family for their histone tail interactions

  Epigenetics & Chromatin · 2020 · 110 citations

  BACKGROUND: Plant homeodomain (PHD) fingers are central "readers" of histone post-translational modifications (PTMs) with > 100 PHD finger-containing proteins encoded by the human genome. Many of the PHDs studied to date bind to unmodified or methylated states of histone H3 lysine 4 (H3K4). Additionally, many of these domains, and the proteins they are contained in, have crucial roles in the regulation of gene expression and cancer development. Despite this, the majority of PHD fingers have gone…

  DOI

• Landscape and selection of vaccine epitopes in SARS-CoV-2

  Genome Medicine · 2021 · 59 citations

  BACKGROUND: T cell stimulation. The rationale for this design was to drive both humoral and cellular immunity with high specificity while avoiding undesired effects such as antibody-dependent enhancement (ADE). METHODS: We explored the set of computationally predicted SARS-CoV-2 HLA-I and HLA-II ligands, examining protein source, concurrent human/murine coverage, and population coverage. Beyond MHC affinity, T cell vaccine candidates were further refined by predicted immunogenicity, sequence con…

  DOI

• Nucleosome conformation dictates the histone code

  eLife · 2024-02-06 · 57 citations

  articleOpen access

  Histone post-translational modifications (PTMs) play a critical role in chromatin regulation. It has been proposed that these PTMs form localized 'codes' that are read by specialized regions (reader domains) in chromatin-associated proteins (CAPs) to regulate downstream function. Substantial effort has been made to define [CAP: histone PTM] specificities, and thus decipher the histone code and guide epigenetic therapies. However, this has largely been done using the reductive approach of isolate…

  PublisherDOI

• An acetylation-mediated chromatin switch governs H3K4 methylation read-write capability

  eLife · 2023 · 56 citations

  H3K4 methylation levels. Together, these observations reveal an acetylation 'chromatin switch' on the H3 tail that modulates read-write accessibility in nucleosomes and resolves the long-standing question of why H3K4me3 levels are coupled with H3 acetylation.

  PublisherDOI

• Specific Inhibition of Orai1-mediated Calcium Signalling Resolves Inflammation and Clears Bacteria in an Acute Respiratory Distress Syndrome Model

  American Journal of Respiratory and Critical Care Medicine · 2023-11-16 · 16 citations

  articleOpen access

  Abstract Rationale Acute respiratory distress syndrome (ARDS) has an unacceptably high mortality rate (35%) and is without effective therapy. Orai1 is a Ca2+ channel involved in store-operated Ca2+ entry (SOCE), a process that exquisitely regulates inflammation. Orai1 is considered a druggable target, but no Orai1-specific inhibitors exist to date. Objectives To evaluate whether ELD607, a first-in-class Orai1 antagonist, can treat ARDS caused by bacterial pneumonia in preclinical models. Methods…

  PublisherOA PDFDOI

Frequent coauthors

• Peter P. Roller

  National Cancer Institute

  142 shared

• Zaneta Nikolovska‐Coleska

  University of Michigan–Ann Arbor

  117 shared

• Shaomeng Wang

  University of Michigan–Ann Arbor

  116 shared

• Chao‐Yie Yang

  University of Michigan–Ann Arbor

  87 shared

• York Tomita

  82 shared

• Jeanne A. Stuckey

  University of Michigan–Ann Arbor

  79 shared

• Brian D. Strahl

  University of North Carolina at Chapel Hill

  69 shared

• Su Qiu

  Hubei University of Chinese Medicine

  66 shared

Labs

• Krajewski LabPI

Similar researchers at University of North Carolina at Chapel Hill

• Michael Rubinsteinh-90
• Ronald Swanstromh-83
• William Marzluffh-78