Gerwald Jogl

About

Gerwald Jogl is an Associate Professor of Biology with a background in Chemistry, holding a Ph.D. from the University of Graz, Austria. His doctoral research involved X-ray and Neutron Diffraction Studies of B12 Coenzymes in both free and enzyme-bound states. He has also completed post-doctoral research as a Research Associate with Liang Tong at Columbia University. His research areas include structural biology, cryo-electron microscopy, antibiotic resistance, CRISPR-Cas activation, retrotransposons, and ribosome function. Jogl's work involves detailed structural and biochemical studies of various biological molecules, including ribosomal components, enzymes involved in biosynthesis, and elements of the CRISPR system, contributing to a deeper understanding of molecular mechanisms and potential therapeutic targets.

Research topics

• Biology
• Computational biology
• Chemistry
• Geometry
• Microbiology
• Organic chemistry
• Mathematical analysis
• Biophysics
• Physics
• Mathematics

Selected publications

• Phase separation of the LINE-1 ORF1 protein is mediated by the N-terminus and coiled-coil domain

  Biophysical Journal · 2021 · 56 citations

  Senior authorCorresponding
  PublisherOA PDFDOI

• High-fidelity, hyper-accurate, and evolved mutants rewire atomic-level communication in CRISPR-Cas9

  Science Advances · 2024-03-06 · 42 citations

  articleOpen access

  The high-fidelity (HF1), hyper-accurate (Hypa), and evolved (Evo) variants of the CRISPR-associated protein 9 (Cas9) endonuclease are critical tools to mitigate off-target effects in the application of CRISPR-Cas9 technology. The mechanisms by which mutations in recognition subdomain 3 (Rec3) mediate specificity in these variants are poorly understood. Here, solution nuclear magnetic resonance and molecular dynamics simulations establish the structural and dynamic effects of high-specificity mut…

  PublisherOA PDFDOI

• Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis

  Scientific Reports · 2020 · 29 citations

  Senior authorCorresponding

  Enterococcus faecalis is a gram-positive organism responsible for serious infections in humans, but as with many bacterial pathogens, resistance has rendered a number of commonly used antibiotics ineffective. Here, we report the cryo-EM structure of the E. faecalis 70S ribosome to a global resolution of 2.8 Å. Structural differences are clustered in peripheral and solvent exposed regions when compared with Escherichia coli, whereas functional centres, including antibiotic binding sites, are simi…

  PublisherOA PDFDOI

• Structural and dynamic insights into the HNH nuclease of divergent Cas9 species

  Journal of Structural Biology · 2021-12-03 · 22 citations

  articleOpen access
  PublisherOA PDFDOI

• Disruption of electrostatic contacts in the HNH nuclease from a thermophilic Cas9 rewires allosteric motions and enhances high-temperature DNA cleavage

  The Journal of Chemical Physics · 2022-12-14 · 17 citations

  articleOpen access

  Allosteric signaling within multidomain proteins is a driver of communication between spatially distant functional sites. Understanding the mechanism of allosteric coupling in large multidomain proteins is the most promising route to achieving spatial and temporal control of the system. The recent explosion of CRISPR-Cas9 applications in molecular biology and medicine has created a need to understand how the atomic level protein dynamics of Cas9, which are the driving force of its allosteric cro…

  PublisherOA PDFDOI

Recent grants

• NIH Grant P20RR015578

  NIH · $9.0M · 2012

• Structural Robustness of Ribosome Functional Centers

  NIH · $4.4M · 2010–2026

• NIH Grant P41RR012408

  NIH · $17.2M · 2013

Frequent coauthors

• Steven T. Gregory

  University of Rhode Island

  56 shared

• George P. Lisi

  Providence College

  55 shared

• Alexandra M. D’Ordine

  Providence College

  54 shared

• Albert E. Dahĺberg

  Providence College

  48 shared

• Hasan DeMi̇rci̇

  Koç University

  46 shared

• Helen B. Belato

  Providence College

  39 shared

• Jinping Luo

  Providence College

  25 shared

• E. Murphy

  Providence College

  22 shared

Labs

• Gerwald Jogl LaboratoryPI

Education

• Ph.D., Chemistry

  University of Graz, Austria

• M.S., Chemistry

  University Graz, Austria

Similar researchers at Brown University

• Weihan Lih-83
• Eric Morrowh-76
• Mark Johnsonh-75