Bruce Applegate

About

Bruce Applegate is a professor in the Department of Food Science with a courtesy appointment in the Department of Biological Sciences at Purdue University. His research areas include microbiology, immunology, and infectious disease, with a focus on the detection of viable foodborne pathogens using bacteriophage, automated extraction of nucleic acids from various matrices, and enumeration of microorganisms such as pathogens and other organisms using quantitative PCR. He also works on the use of bioreporters in bioelectronics, metabolic engineering, detection of problematic microorganisms in industrial environments, construction of recombinant bacterial strains to rapidly evaluate antimicrobial products, and microbial ecology. His work aims to improve food safety and microbial detection methods, contributing to advancements in food microbiology and bioelectronics.

Research topics

• Biology
• Microbiology
• Food science
• Chemistry
• Biochemistry
• Materials science
• Nanotechnology

Selected publications

• Receptor-targeted engineered probiotics mitigate lethal Listeria infection

  Nature Communications · 2020 · 95 citations

  dendritic cells and natural killer cells. Engineering a probiotic strain with an adhesion protein from a non-pathogenic bacterium provides a new paradigm to exclude pathogens and amplify their inherent health benefits.

  PublisherOA PDFDOI

• Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment

  Biosensors · 2022 · 82 citations

  Foodborne microorganisms are an important cause of human illness worldwide. Two-thirds of human foodborne diseases are caused by bacterial pathogens throughout the globe, especially in developing nations. Despite enormous developments in conventional foodborne pathogen detection methods, progress is limited by the assay complexity and a prolonged time-to-result. The specificity and sensitivity of assays for live pathogen detection may also depend on the nature of the samples being analyzed and t…

  PublisherOA PDFDOI

• Ultrasound combined with slightly acidic electrolyzed water thawing of mutton: Effects on physicochemical properties, oxidation and structure of myofibrillar protein

  Ultrasonics Sonochemistry · 2023-01-23 · 58 citations

  articleOpen access

  The effects of air thawing (AT), water immersion thawing (WT), microwave thawing (MT) and ultrasound combined with slightly acidic electrolyzed water thawing (UST) on the myofibrillar protein (MP) properties (surface hydrophobicity, solubility, turbidity, particle size and zeta potential), protein oxidation (carbonyl content and sulfhydryl content) and structure (primary, secondary and tertiary) of frozen mutton were investigated in comparison with fresh mutton (FM). The solubility and turbidity…

  PublisherDOI

• Penetration and Microbial Inactivation by High Voltage Atmospheric Cold Plasma in Semi-Solid Material

  Food and Bioprocess Technology · 2020 · 37 citations

  PublisherDOI

• Bacteriophage-based biocontrol technology to enhance the efficiency of wastewater treatment and reduce targeted bacterial biofilms

  The Science of The Total Environment · 2022-12-08 · 35 citations

  review
  PublisherDOI

Frequent coauthors

• Gary S. Sayler

  University of Tennessee at Knoxville

  28 shared

• Lisa J. Mauer

  13 shared

• Michael L. Simpson

  13 shared

• Steven Ripp

  University of Tennessee at Knoxville

  12 shared

• Ernest R. Blatchley

  11 shared

• Ronald F. Turco

  11 shared

• John Sanseverino

  10 shared

• Caula A. Beyl

  University of Tennessee at Knoxville

  9 shared

Similar researchers at Purdue University

• R. Claudio Aguilarh-32
• Ximena Bernalh-32
• Beatriz Castroh-25