Beth A Gibson

Anthrax lethal toxin paralyzes actin‐based motility by blocking Hsp27 phosphorylation

The EMBO Journal · 2007-04-19 · 88 citations

Depolymerization of Actin Filaments by Profilin

Journal of Biological Chemistry · 2003-06-29 · 64 citations

Profilin interacts with the barbed ends of actin filaments and is thought to facilitate in vivo actin polymerization. This conclusion is based primarily on in vitro kinetic experiments using relatively low concentrations of profilin (1–5 μm). However, the cell contains actin regulatory proteins with multiple profilin binding sites that potentially can attract millimolar concentrations of profilin to areas requiring rapid actin filament turnover. We have studied the effects of higher concentratio…

Gelsolin mediates calcium-dependent disassembly of<i>Listeria</i>actin tails

Proceedings of the National Academy of Sciences · 2005-01-25 · 41 citations

The role of intracellular Ca2+ in the regulation of actin filament assembly and disassembly has not been clearly defined. We show that reduction of intracellular free Ca2+ concentration ([Ca2+]i) to <40 nM in Listeria monocytogenes-infected, EGFP-actin-transfected Madin-Darby canine kidney cells results in a 3-fold lengthening of actin filament tails. This increase in tail length is the consequence of marked slowing of the actin filament disassembly rate, without a significant change in assembly…

A CapG gain‐of‐function mutant reveals critical structural and functional determinants for actin filament severing

The EMBO Journal · 2006-09-14 · 30 citations

Electronic excitation transfer in the complex of lumazine protein with bacterial bioluminescence intermediates

Biochemistry · 1991-07-16 · 28 citations

Fluorescence dynamics measurements have been made on the bioluminescence reaction intermediates using Photobacterium leiognathi, Vibrio fischeri, and Vibrio harveyi luciferases, both alone and in mixtures with Photobacterium phosphoreum lumazine protein. Each luciferase produces a "fluorescent transient" intermediate on reaction with the bioluminescence substrates, FMNH2, tetradecanal, and O2, and all have a fluorescence quantum yield about 0.3, with a predominant lifetime around 10 ns. The P. l…