Preoperative differentiation of primary vs. metastatic lumbar spine tumors: development and external validation of a multiparametric MRI-based radiomics nomogram

Frontiers in Oncology · 2026-04-14

Objective: To develop and externally validate a multiparametric magnetic resonance imaging (MRI)-based radiomics nomogram for preoperative differentiation of primary from metastatic lumbar spine tumors. Methodology: 200 patients were divided into training (n=100) and independent external validation (n=100) cohorts. Radiomics features from T1WI, T2WI, FS-T2WI were filtered and reduced via LASSO to construct Radscore; a combined nomogram integrating Radscore and clinical variables was evaluated. Results: The combined nomogram demonstrated excellent discriminatory ability in the independent external validation cohort, with an area under the curve (AUC) of 0.921 (95% confidence interval [CI]: 0.838-0.970). Its performance was significantly superior to that of the clinical variables-only model (AUC: 0.732, P < 0.001) and the Radscore-only model (AUC: 0.880, P = 0.028), achieving a sensitivity of 85% and a specificity of 87%. Univariate and multivariate logistic regression analyses identified the Radscore, age > 60 years, and serum alkaline phosphatase (ALP) > 120 U/L as independent predictors for differentiating primary from metastatic lumbar spine tumors. The nomogram exhibited good calibration (Hosmer-Lemeshow test, P = 0.62). Decision curve analysis (DCA) confirmed its clinical utility by showing a higher net benefit across a wide range of threshold probabilities compared to default strategies. Conclusion: A radiomics nomogram integrating multiparametric MRI features and key clinical factors was successfully developed and externally validated. It serves as an effective, non-invasive auxiliary tool for preoperative differentiation of primary from metastatic lumbar spine tumors, with potential for clinical translation.

Oral iron sulfide prevents acute alcohol intoxication by initiating the endogenous multienzymatic antioxidant defense system

Science Advances · 2025-01-17 · 7 citations

Acute alcohol intoxication could cause multiorgan damage, including nervous, digestive, and cardiovascular systems, and in particular, irreversible damage to the brain and liver. Emerging studies have revealed that the endogenous multienzymatic antioxidant defense system (MEAODS) plays a central role in preventing oxidative stress and other toxicological compounds produced by alcohol. However, few available drugs could quickly regulate MEAODS. Herein, we report a nanosized iron sulfide (nFeS) that can rapidly release polysulfide species in gastric juice. The released hydrogen polysulfide activates the Keap1/Nrf2 pathway via S-persulfidation of cysteine residues in Keap1, which promotes the expression of antioxidant enzymes and glutathione synthesis-related enzymes, thus potentiating MEAODS. Results indicate that the activated MEAODS not only alleviates oxidative stress and inflammation in the brain and liver but also mitigates movement dysfunction after only 2.5 hours of oral nFeS treatment. Collectively, this study provides a MEAODS-regulated strategy with nFeS and may aid the prevention of acute alcoholic injury.

Retinal proteomic analysis reveals ON/OFF visual stimulation-specific changes in a Guinea pig myopia model

Experimental Eye Research · 2025-08-25 · 2 citations

Congenital Alopecia and Cataracts Associated With a Novel <scp> <i>EBP</i> </scp> Variant: A Phenocopy of Lanosterol Synthase Deficiency

The Journal of Dermatology · 2025-09-16

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.

Advancing e-assessment for learning in the primary EFL writing classroom: the role of collaborative teacher professional learning

Asia Pacific Journal of Education · 2025-03-04 · 1 citations

Recrystallization behaviors of AlMgScZr alloys with different Al3(Sc,Zr) dispersoid distribution

Materials Characterization · 2025-07-08 · 11 citations

Effect of Music Therapy Integrated with ORTCC Model-Based Nursing on Patients Undergoing Vacuum Sealing Drainage in Surgical Settings

Noise and Health · 2025-11-01

OBJECTIVE: This study aimed to analyse the effects of music therapy combined with the Objective-Rules-Training-Check-Culture (ORTCC) model-based nursing in patients undergoing vacuum sealing drainage (VSD) for surgical wounds. METHODS: In this retrospective cohort study, clinical data of 132 patients from Cangzhou Central Hospital receiving VSD for hand and foot surgeries between June 2022 and June 2023 were retrospectively analysed. In accordance with actual nursing protocols, participants were allocated to either the ORTCC group (n = 67), receiving systematic ORTCC model-based nursing, or the combination group (n = 65), receiving additional music therapy alongside ORTCC nursing. Outcomes included fear of disease progression [Fear of Progression Questionnaire-Short Form (FoP-Q-SF)], mood states [Brief Profile of Mood States (BPOMS)], quality of life [Generic Quality of Life Inventory-74 (GQOLI-74)], compliance rates, satisfaction scores and complication incidence. RESULTS: No significant intergroup differences were observed in wound healing time, pain scores and complication rates (P > 0.05). Both groups exhibited reduced FoP-Q-SF and BPOMS scores after care compared with baseline, with significantly lower scores in the combination group than in the ORTCC group (P < 0.05). All GQOLI-74 domain scores increased after care in both groups, but they were significantly higher in the combination group (P < 0.05). The combination group demonstrated superior nursing compliance rates and satisfaction scores (P < 0.05). CONCLUSION: Surgical patients undergoing VSD and receiving combined music therapy and ORTCC model-based nursing exhibited significantly reduced fear of disease progression, mood improvement, enhanced quality of life and superior compliance and satisfaction, though no additional advantages were observed in physiological wound healing.

Mitochondrial unfolded protein response (UPR ^mt ) as novel therapeutic targets for neurological disorders

Journal of Cerebral Blood Flow & Metabolism · 2025-05-15 · 5 citations

Neurological disorders, including brain cancer, neurodegenerative diseases and ischemic/reperfusion injury, pose a significant threat to global human health. Due to the high metabolic demands of nerve cells, mitochondrial dysfunction is a critical feature of these disorders. The mitochondrial unfolded protein response (UPR mt ) is an evolutionarily conserved mitochondrial response, which is critical for maintaining mitochondrial and energetic homeostasis under stress. Previous studies have found that UPR mt participates in diverse physiological processes especially metabolism and immunity. Currently, increasing evidence suggest that targeted regulation of UPR mt can also effectively delay the progression of neurological diseases and improve patients’ prognosis. This review provides a comprehensive overview of UPR mt in the context of neurological diseases, with a particular emphasis on its regulatory functions. Additionally, we summarize the mechanistic insights into UPR mt in neurological disorders as investigated in preclinical studies, as well as its potential as a therapeutic target in the clinical management of neurological tumors. By highlighting the importance of UPR mt in the complex processes underlying neurological disorders, this review aims to bridge current knowledge gaps and inspire novel therapeutic strategies for these conditions.

2D MOF Nanozyme‐Enhanced Impedance Detector for Synergistic Biofilm Detection and Removal in Bioelectrochemical Systems

Chemistry - A European Journal · 2025-05-01 · 3 citations

The formation of biofilm has become a significant influencing factor to microbial fuel cells (MFCs). Developing effective biofilm detection means are highly imperative for improving the efficiency of MFCs. In this study, we propose a 2D metal-organic framework (2D Cu-BDC) nanozyme-based impedance detector for the simultaneous detection and removal of bacterial biofilms. The promising induction interface features good electron transfer and biofilm attachment properties, realizing sensitive biofilm detection. The impedance response change on the functionalized 2D Cu-BDC detector was 3.5 times higher for Gram-positive bacteria biofilm and 2.7 times higher for Gram-negative bacteria biofilm compared to bare electrodes. In addition, the 2D Cu-BDC nanozyme demonstrates robust peroxidase-like activity, enabling efficient biofilm degradation at low H2O2 concentration, and further allowing for real-time monitoring of the biofilm elimination process. This study provides a novel approach to using MOF-based biosensors for the simultaneous detection and removal of biofilm in MFCs.

Tea seed-derived leucoside attenuates sepsis via inhibition of TLR4-MD2 complex formation

Biochemical Pharmacology · 2025-10-24

Sepsis, a life-threatening condition driven by dysregulated inflammation, remains a major clinical challenge due to high mortality rates and limited therapeutic options. This study investigates the anti-inflammatory properties of Leucoside, a natural flavonoid isolated from tea seed extract, and its potential as a therapeutic agent for sepsis. Using a bacterial infection-induced septic mouse model and lipopolysaccharide (LPS)-activated macrophages, we demonstrated that Leucoside significantly improves survival rates, reduces hypothermia, and attenuates organ damage by suppressing systemic inflammation. Mechanistically, network pharmacology and molecular docking identified Toll-like receptor 4 (TLR4) as a primary target of Leucoside. Biochemical and structural analyses revealed that Leucoside competitively binds to conserved positively charged residues in the B patch of TLR4, specifically Lys263 and Arg337, forming a spatial barrier that inhibits the formation of the myeloid differentiation protein 2 (MD2)-TLR4 complex and subsequent nuclear factor kappa-B (NF-κB) signaling. This inhibition was further validated through co-immunoprecipitation assays, which showed a reduced effect on the TLR4-MD2 complex dissociation when Lys263 and Arg337 were mutated. These findings highlight Leucoside as a novel TLR4 inhibitor with significant potential for treating sepsis and other TLR4-mediated inflammatory diseases. By elucidating its mechanism of action, this study provides a foundation for developing targeted therapies to address the unmet clinical needs in sepsis management.