Thomas Neufeld

About

Thomas Neufeld, PhD, is a professor affiliated with the Genetics, Cell Biology & Development department at the University of Minnesota Medical School. His professional profile is accessible through the university's website, indicating his role within the academic community. The information provided does not specify his research focus, background, or key contributions, but it confirms his position as a faculty member involved in genetics, cell biology, and development.

Research topics

• Medicine
• Genetics
• Biology
• Art
• Pediatrics
• Cell biology
• Internal medicine
• Philosophy
• Gerontology
• Theology

Selected publications

• ULK1 induces autophagy by phosphorylating Beclin-1 and activating VPS34 lipid kinase

  Nature Cell Biology · 2013-05-19 · 1529 citations

  articleOpen access
  PublisherOA PDFDOI

• Bafilomycin A1 disrupts autophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome fusion

  Autophagy · 2015-07-09 · 789 citations

  articleOpen accessSenior authorCorresponding

  Autophagosome-lysosome fusion and autolysosome acidification constitute late steps in the autophagic process necessary to maintain functional autophagic flux and cellular homeostasis. Both of these steps are disrupted by the V-ATPase inhibitor bafilomycin A1, but the mechanisms potentially linking them are unclear. We recently revisited the role of lysosomal acidification in autophagosome-lysosome fusion, using an in vivo approach in Drosophila. By genetically depleting individual subunits of th…

  PublisherOA PDFDOI

• Autophagosome–lysosome fusion is independent of V-ATPase-mediated acidification

  Nature Communications · 2015-05-11 · 432 citations

  articleOpen accessSenior author

  The ATP-dependent proton pump V-ATPase ensures low intralysosomal pH, which is essential for lysosomal hydrolase activity. Based on studies with the V-ATPase inhibitor BafilomycinA1, lysosomal acidification is also thought to be required for fusion with incoming vesicles from the autophagic and endocytic pathways. Here we show that loss of V-ATPase subunits in the Drosophila fat body causes an accumulation of non-functional lysosomes, leading to a block in autophagic flux. However, V-ATPase-defi…

  PublisherOA PDFDOI

• Dietary sugar promotes systemic TOR activation in Drosophila through AKH-dependent selective secretion of Dilp3

  Nature Communications · 2015-04-17 · 187 citations

  articleOpen accessSenior author

  Secreted ligands of the insulin family promote cell growth and maintain sugar homeostasis. Insulin release is tightly regulated in response to dietary conditions, but how insulin-producing cells (IPCs) coordinate their responses to distinct nutrient signals is unclear. Here we show that regulation of insulin secretion in Drosophila larvae has been segregated into distinct branches—whereas amino acids promote the secretion of Drosophila insulin-like peptide 2 (Dilp2), circulating sugars promote t…

  PublisherOA PDFDOI

• Membrane remodeling by the PX-BAR protein SNX18 promotes autophagosome formation

  The Journal of Cell Biology · 2013-07-22 · 178 citations

  articleOpen access

  The membrane remodeling events required for autophagosome biogenesis are still poorly understood. Because PX domain proteins mediate membrane remodeling and trafficking, we conducted an imaging-based siRNA screen for autophagosome formation targeting human PX proteins. The PX-BAR protein SNX18 was identified as a positive regulator of autophagosome formation, and its Drosophila melanogaster homologue SH3PX1 was found to be required for efficient autophagosome formation in the larval fat body. We…

  PublisherOA PDFDOI

Recent grants

• NIH Grant R01GM062509

  NIH · $3.8M · 2015

Frequent coauthors

• Elaine C.M. Silva-Zacarin

  21 shared

• Anna Nogalska

  Broad Center

  20 shared

• Wen H. Yu

  Wuhan University of Technology

  20 shared

• Dieter Willbold

  Heinrich Heine University Düsseldorf

  19 shared

• Gerald M. Rubin

  Howard Hughes Medical Institute

  19 shared

• Xuejun Jiang

  18 shared

• Frank C. Dorsey

  17 shared

• Frank Lafont

  16 shared

Similar researchers at University of Minnesota

• Yasuhiko Kawakamih-60
• Heewon Leeh-59
• R. Scott McIvorh-51