Mary L. Kraft

About

Professor Mary L. Kraft is an Associate Professor in the Department of Chemical & Biomolecular Engineering at the University of Illinois at Urbana-Champaign. She is also an affiliate in the Department of Chemistry and the Center for Biophysics and Quantitative Biology at the same institution. Her professional contact information includes an office phone number and email address at the University of Illinois. The Kraft research group, which she leads, is affiliated with both the Department of Chemistry and the Center for Biophysics and Quantitative Biology, indicating a multidisciplinary approach to research. The group includes graduate and undergraduate students, reflecting an active research and teaching environment under her guidance.

Research topics

• Biology
• Chemistry
• Biophysics
• Computer Science
• Biochemistry
• Chromatography
• Physics
• Cell biology
• Biological system
• Nanotechnology

Selected publications

• High-Resolution Secondary Ion Mass Spectrometry Analysis of Cell Membranes

  Analytical Chemistry · 2019-12-19 · 31 citations

  articleSenior authorCorresponding

  This Feature describes the use a Cameca NanoSIMS instrument for directly imaging specific lipid and protein species in the plasma membranes of mammalian cells with approximately 100 nm-lateral resolution and discusses how these analyses have already begun to transform fundamental concepts in the field of membrane biology. Secondary ion generation is discussed with emphasis on the constraints that affect the detection and identification of membrane components, and then the sample preparation meth…

  PublisherDOI

• Toward Understanding the Subcellular Distributions of Cholesterol and Sphingolipids Using High-Resolution NanoSIMS Imaging

  Accounts of Chemical Research · 2023 · 9 citations

  Senior authorCorresponding

  Characterizing the subcellular distributions of biomolecules of interest is a basic inquiry that helps inform on the potential roles of these molecules in biological functions. Presently, the functions of specific lipid species and cholesterol are not well understood, partially because cholesterol and lipid species of interest are difficult to image with high spatial resolution but without perturbing them. Because cholesterol and lipids are relatively small and their distributions are influenced…

  PublisherDOI

• Depth Correction of 3D NanoSIMS Images Shows Intracellular Lipid and Cholesterol Distributions while Capturing the Effects of Differential Sputter Rate

  ACS Nano · 2022 · 9 citations

  Senior authorCorresponding

  N-sphingolipids. Accurate 3D NanoSIMS images of intracellular component distributions may now be produced without requiring correlated analyses with separate instruments or the assumption of a constant sputter rate. This will allow visualization of the subcellular distributions of lipids, metabolites, drugs, and nanoparticles in 3D, information pivotal to understanding and treating disease.

  PublisherDOI

• Identification of the Differentiation Stages of Living Cells from the Six Most Immature Murine Hematopoietic Cell Populations by Multivariate Analysis of Single-Cell Raman Spectra

  Analytical Chemistry · 2022-08-24 · 7 citations

  articleOpen accessSenior authorCorresponding

  Efforts to expand hematopoietic stem and progenitor cells (HSPCs) in vitro are motivated by their use in the treatment of leukemias and other blood and immune system diseases. The combinations of extrinsic cues within the hematopoietic stem cell (HSC) niche that lead to HSC fate decisions remain unknown. New noninvasive and location-specific techniques are needed to enable identification of the differentiation stages of individual hematopoietic cells on biomaterial microarray screening platforms…

  PublisherOA PDFDOI

• Exploring the maturation of a monocytic cell line using self-organizing maps of single-cell Raman spectra

  Biointerphases · 2020-07-01 · 6 citations

  articleOpen accessSenior author

  Phorbol myristate acetate (PMA)-differentiated THP-1 cells are routinely used in lieu of primary macrophages to study macrophage polarization during host-pathogen interactions and disease progression. The phenotypes of THP-1 macrophages are influenced by the level and duration of PMA stimulation and possibly also by the presence of adhesion factors. Here, we use self-organizing maps (SOMs) of single-cell Raman spectra to probe the effects of PMA stimulation conditions and adhesion factors on THP…

  PublisherOA PDFDOI

Recent grants

• High-Resolution Secondary Ion Mass Spectrometry for Chemically Imaging Cell Membrane Organization

  NSF · $400k · 2015–2019

• NIH Grant R21HL132642

  NIH · $398k · 2019

• NIH Grant R01GM109888

  NIH · $1.5M · 2019

Frequent coauthors

• Peter K. Weber

  Lawrence Livermore National Laboratory

  38 shared

• Joshua Zimmerberg

  24 shared

• Brittney Gorman

  24 shared

• I. D. Hutcheon

  Lawrence Livermore National Security

  20 shared

• Steven G. Boxer

  Stanford University

  19 shared

• Brendan A.C. Harley

  University of Illinois Urbana-Champaign

  17 shared

• Jessica F. Frisz

  University of Illinois Urbana-Champaign

  14 shared

• Kaiyan Lou

  Northwestern University

  12 shared

Labs

• Kraft GroupPI

  Not provided

Education

• PhD, Chemistry

  University of Illinois Urbana-Champaign

  2003

• BS, Biochemistry

  Univ of IL Urbana-Champaign

  1998

Awards & honors

• Excellence in Advising Award, College of Engineering, Univer…
• Career Award at the Scientific Interface, Burroughs Wellcome…
• Kirschstein Postdoctoral Fellow, National Institutes of Heal…
• School of Chemical Sciences Excellence in Teaching (2014-201…
• American Vacuum Society (AVS) Prairie Chapter Early-Career R…

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