About

Laurianne Van Landeghem is an Associate Professor at NC State University's College of Veterinary Medicine, specifically within the Department of Molecular Biomedical Sciences. The page does not provide specific details about her research focus, background, or key contributions.

Research topics

• Biology
• Cell biology
• Medicine
• Endocrinology
• Internal medicine

Selected publications

• Protocol to culture enteric glial cells from the submucosal and myenteric plexi of neonatal and juvenile pig colons

  STAR Protocols · 2024-05-18 · 4 citations

  articleOpen accessSenior authorCorresponding

  Here, we present our protocol to culture enteric glial cells from the submucosal and myenteric plexus of neonatal and juvenile pig colons. We describe steps for colon isolation, microdissection, and enzymatic and mechanical dissociation. We include procedures for passaging and analyzing cell yield, freeze/thaw efficiency, and purity. This protocol allows for the generation of primary cultures of enteric glial cells from single-cell suspensions of microdissected layers of the colon wall and can be used to culture enteric glia from human colon specimens. For complete details on the use and execution of this protocol, please refer to Ziegler et al.1

  PublisherDOI

• Prebiotic galactooligosaccharide improves piglet growth performance and intestinal health associated with alterations of the hindgut microbiota during the peri-weaning period

  Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 2024-06-13 · 10 citations

  articleOpen access

  BACKGROUND: Weaning stress reduces growth performance and health of young pigs due in part to an abrupt change in diets from highly digestible milk to fibrous plant-based feedstuffs. This study investigated whether dietary galactooligosaccharide (GOS), supplemented both pre- and post-weaning, could improve growth performance and intestinal health via alterations in the hindgut microbial community. METHODS: Using a 3 × 2 factorial design, during farrowing 288 piglets from 24 litters received either no creep feed (FC), creep without GOS (FG-) or creep with 5% GOS (FG+) followed by a phase 1 nursery diet without (NG-) or with 3.8% GOS (NG+). Pigs were sampled pre- (D22) and post-weaning (D31) to assess intestinal measures. RESULTS: Creep fed pigs grew 19% faster than controls (P < 0.01) prior to weaning, and by the end of the nursery phase (D58), pigs fed GOS pre-farrowing (FG+) were 1.85 kg heavier than controls (P < 0.05). Furthermore, pigs fed GOS in phase 1 of the nursery grew 34% faster (P < 0.04), with greater feed intake and efficiency. Cecal microbial communities clustered distinctly in pre- vs. post-weaned pigs, based on principal coordinate analysis (P < 0.01). No effects of GOS were detected pre-weaning, but gruel creep feeding increased Chao1 α-diversity and altered several genera in the cecal microbiota (P < 0.05). Post-weaning, GOS supplementation increased some genera such as Fusicatenibacter and Collinsella, whereas others decreased such as Campylobacter and Frisingicoccus (P < 0.05). Changes were accompanied by higher molar proportions of butyrate in the cecum of GOS-fed pigs (P < 0.05). CONCLUSIONS: Gruel creep feeding effectively improves suckling pig growth regardless of GOS treatment. When supplemented post-weaning, prebiotic GOS improves piglet growth performance associated with changes in hindgut microbial composition.

  PublisherOA PDFDOI

• Mini-Review: Enteric glia of the tumor microenvironment: An affair of corruption

  Neuroscience Letters · 2023-08-10 · 4 citations

  reviewOpen accessSenior authorCorresponding
  PublisherOA PDFDOI

• Supplementary Figure 1 from Cytokine Induction of Tumor Necrosis Factor Receptor 2 Is Mediated by STAT3 in Colon Cancer Cells

  2023-04-03

  preprintOpen access

  &lt;p&gt;PDF file - 193K&lt;/p&gt;

  PublisherDOI

• Supplementary Tables 1-3 from Cytokine Induction of Tumor Necrosis Factor Receptor 2 Is Mediated by STAT3 in Colon Cancer Cells

  2023-04-03

  supplementary-materialsOpen access

  &lt;p&gt;PDF file - 52K&lt;/p&gt;

  PublisherOA PDFDOI

• 257 Effects of Prebiotic Galactooligosaccharide (GOS) on Hindgut Microbial Composition of Pigs During the Peri-Weaning Period

  Journal of Animal Science · 2023-11-06

  articleOpen access

  Abstract Galactooligosaccharide (GOS) is a prebiotic containing beta-linked galactose oligomers of 2 to 8 units. Previously, GOS positively impacted post-weaning growth performance and altered jejunal morphology. In this study, GOS-enriched whey permeate (Milk Specialties Global, Eden Prairie MN) was supplemented to piglets in farrowing and nursery phases. To maximize pre-weaning GOS intake, novel gruel creep feeders were used. Piglets from 24 litters received either no creep feed (NC), creep without GOS (CG-) or creep with 5.0% GOS (CG+) followed by a phase 1 nursery diet without (NG-) or with 3.8% GOS (NG+). At d 22 (pre-weaning) and d 31 (post-weaning), 6 pigs per treatment were euthanized for sample collection. DNA was extracted from cecal and rectal swabs for downstream 16S rRNA sequencing via Illumina MiSeq (Zymo Research Corp, Irvine CA). An amplicon sequence variant (ASV) table was created using the DADA2 pipeline in R and taxonomy assignment was performed using the SILVA database. Average sequencing depth after filtering was 138,323 and low sequencing counts were discarded based on 20% prevalence. Data were analyzed using the lmer and adonis procedures in RStudio ver. 4.2.2. Analysis of 16S rRNA genes did not detect pre- or post-weaning GOS effects on Chao1, Simpson, or Shannon alpha diversity measures (P &amp;gt; 0.1). Similarly, no pre-weaning GOS effects were detected in Principal Coordinates Analysis (PCoA) beta diversity plots (P &amp;gt; 0.1); however, pigs fed GOS post-weaning segregated from controls (P &amp;lt; 0.01). The relative abundance of several genera including Fusicatenbacter and Collinsella increased by 2.0 and 1.3-fold, respectively, in the cecum of NG+ pigs, whereas, Bacteroides decreased by 3.8-fold (FDR, P &amp;lt; 0.05). In the rectum, relative abundance of Corprococcus decreased by 2.0-fold and Fusicatenbacter increased by 1.5-fold in NG+ pigs compared with NG- pigs (FDR, P &amp;lt; 0.05). Collectively, these genera in the cecum and rectum comprise of less than 3% of the total community. In contrast, lactobacillus in the cecum (33%) and rectum (21%) had a large relative abundance but was unaffected by treatment. We conclude that supplementation of GOS in the post-weaning phase 1 nursery diet alters the hindgut microbial community and may contribute to improvements in growth performance.

  PublisherOA PDFDOI

• Neonatal Enteric Glia Enhance Intestinal Epithelial Restitution in vitro Following Exposure to Sterile Colonic Luminal Content of Mature but not Neonatal Pigs

  Physiology · 2023-05-01

  article

  Neonates exhibit significantly poorer outcomes from damage to the intestinal epithelium following ischemia for unknown reasons. In our pig model, we recently discovered an age-dependent epithelial restitution defect in ischemia-injured neonatal intestine that is rescued by application of homogenized juvenile mucosa, but the mechanism remains unknown. Enteric glial cells (EGC) have been shown to promote epithelial restitution following injury via paracrine signaling and are abundant in the intestinal mucosa of juvenile but not neonatal pigs. Co-culture studies have shown that pig EGC enhance restitution in epithelial monolayers following scratch wounding. In mice, postnatal maturation and maintenance of the EGC network is purportedly driven by colonization of gut microbiota. Furthermore, weaning of neonates leads to drastic shifts in the dynamics and populations of these gut microbiota. Therefore, we believe that changes to pig intestinal microbiota during weaning may play a key role in EGC maturation required for proper epithelial restitution. We hypothesized that treatment with mature (&gt;6 weeks of age), but not neonatal (2 weeks of age), luminal content would improve restitution of neonatal epithelial monolayers co-cultured with neonatal EGC. We compared the effect of sterile-filtered neonatal or mature luminal content addition to the apical chamber on scratch wound restitution in neonatal porcine IPEC-J2 monolayers in monoculture or co-culture with primary porcine neonatal submucosal EGC using a transwell system (n = 9). Our results support a significant effect of luminal content treatment in the presence of EGC (P=0.0085). Specifically, mature luminal content treatment enhanced IPEC-J2 wound closure in co-culture with EGC but not in IPEC-J2 monoculture (P=0.0156). EGC co-culture alone or treated with neonatal lumen content did not affect restitution. Both neonatal and mature luminal content treatment decreased restitution in monoculture, but this effect was not significant. These data suggest that EGC provide secretory signals to the intestinal epithelium that promote repair in response to a component of mature luminal content, which we believe to be microbial products. Future work aims to determine what is driving these differences in restitution by analyzing neonatal and mature luminal secretomes. NIH 5 T35 OD 11070-12; NIH K01 OD 028207; NIH P30 DK 034987; NIH-NICHD R01 HD095876; USDA-NIFA VMCG-0065. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

  PublisherDOI

• PSI-20 Effects of Prebiotic Galactooligosaccharide (Gos) on Piglet Growth and Jejunal Morphology During the Peri-Weaning Period

  Journal of Animal Science · 2023-10-28

  articleOpen access

  Abstract Galactooligosaccharide (GOS) is a specific prebiotic that is enzymatically synthesized from lactose to form beta-linked oligosaccharides containing 2-8 galactose units. In this study, GOS-enriched whey permeate (Milk Specialties Global, Eden Prairie MN) was supplemented to piglets in lactation and nursery phases. To maximize pre-weaning GOS intake, novel gruel creep feeders were utilized. Using a 3x2 factorial design, piglets from 24 litters received either no creep feed (NC), creep without GOS (CG-) or creep with 5% GOS (CG+) followed by a phase 1 nursery diet without (NG-) or with 3.8% GOS (NG+). After 1 week, pigs were fed common phase 2 and phase 3 diets. At d 22 (pre-weaning) and d 31 (post-weaning), 6 pigs per treatment were euthanized for intestinal measurements. Pre-weaning, creep fed pigs grew 19% faster than controls (P &amp;lt; 0.01) but GOS effects were not detected (P &amp;gt; 0.1). In contrast, pigs fed GOS in phase 1 grew 34 % faster than controls (P &amp;lt; 0.04), irrespective of creep treatment (interaction P &amp;gt; 0.1), and with corresponding greater intakes (P &amp;lt; 0.06). These GOS effects were sustained for overall nursery performance. Furthermore, overall ADG of CG+ piglets in the nursery tended to be greatest (P = 0.09), gaining 361g/d, followed by NC (324 g/d) and CG- (310 g/d) treatments (Table 1). No effects on jejunal morphology were detected at d 22, although there was an effect of age with decreased villi length, villus area, villi:crypt ratio and increased crypt depth at d 31 (P &amp;lt; 0.01). Supplementation of GOS in phase 1 increased villus length (36%) and area (51%) but only in pigs previously fed the control creep (CG-) diet (interaction, P &amp;lt; 0.01). Treatment effects on cecal pH and VFA concentrations were not detected, although there was an effect of age with a decrease in pH (P &amp;lt; 0.01) and increase in propionate and butyrate concentrations post-weaning (P &amp;lt; 0.01). We conclude that gruel creep feeding increases weight gain regardless of GOS treatment and that nursery growth and intestinal morphology are improved by post-weaning GOS supplementation. Funded in part by Milk Specialties Global, USDA-NIFA 2022-67015-37125 and Hatch 1016618.

  PublisherOA PDFDOI

• Data from Cytokine Induction of Tumor Necrosis Factor Receptor 2 Is Mediated by STAT3 in Colon Cancer Cells

  2023-04-03

  preprintOpen access

  &lt;div&gt;Abstract&lt;p&gt;The IL-6/STAT3 and TNFα/NFκB pathways are emerging as critical mediators of inflammation-associated colon cancer. TNF receptor (TNFR) 2 expression is increased in inflammatory bowel diseases, the azoxymethane/dextran sodium sulfate (AOM/DSS) model of colitis-associated cancer, and by combined interleukin (IL) 6 and TNFα. The molecular mechanisms that regulate TNFR2 remain undefined. This study used colon cancer cell lines to test the hypothesis that IL-6 and TNFα induce TNFR2 via STAT3 and/or NFκB. Basal and IL-6 + TNFα–induced TNFR2 were decreased by pharmacologic STAT3 inhibition. NFκB inhibition had little effect on IL-6 + TNFα–induced TNFR2, but did inhibit induction of endogenous IL-6 and TNFR2 in cells treated with TNFα alone. Chromatin immunoprecipitation (ChIP) revealed cooperative effects of IL-6 + TNFα to induce STAT3 binding to a −1,578 STAT response element in the &lt;i&gt;TNFR2&lt;/i&gt; promoter but no effect on NFκB binding to consensus sites. Constitutively active STAT3 was sufficient to induce TNFR2 expression. Overexpression of SOCS3, a cytokine-inducible STAT3 inhibitor, which reduces tumorigenesis in preclinical models of colitis-associated cancer, decreased cytokine-induced TNFR2 expression and STAT3 binding to the −1,578 STAT response element. SOCS3 overexpression also decreased proliferation of colon cancer cells and dramatically decreased anchorage-independent growth of colon cancer cells, even cells overexpressing TNFR2. Collectively, these studies show that IL-6- and TNFα-induced TNFR2 expression in colon cancer cells is mediated primarily by STAT3 and provide evidence that TNFR2 may contribute to the tumor-promoting roles of STAT3. &lt;i&gt;Mol Cancer Res; 9(12); 1718–31. ©2011 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;

  PublisherDOI

• Enteric glial cell network function is required for epithelial barrier restitution following intestinal ischemic injury in the early postnatal period

  American Journal of Physiology-Gastrointestinal and Liver Physiology · 2023-12-26 · 10 citations

  articleOpen access

  This study refines a powerful translational pig model, defining an age-dependent relationship between enteric glia and the intestinal epithelium during intestinal ischemic injury and confirming an important role for enteric glial cell (EGC) activity in driving mucosal barrier restitution. This study suggests that targeting the enteric glial network could lead to novel interventions to improve recovery from intestinal injury in neonatal patients.

  PublisherOA PDFDOI

Recent grants

• Enteric Glia is New Biological Target to Block Drug Resistance in Colon Cancer

  NIH · $1.3M · 2023–2028

Frequent coauthors

• Michel Neunlist

  Nantes Université

  43 shared

• Mélissa Touvron

  North Carolina State University

  30 shared

• Anthony T. Blikslager

  North Carolina State University

  21 shared

• P. Kay Lund

  21 shared

• Amanda L. Ziegler

  North Carolina State University

  21 shared

• Émilie Duchalais

  Centre Hospitalier Universitaire de Nantes

  19 shared

• Jack Odle

  North Carolina State University

  18 shared

• Pascal Derkinderen

  Centre Hospitalier Universitaire de Nantes

  18 shared

Education

• Ph.D., Comparative Medicine

  North Carolina State University

  2009

• M.S., Comparative Medicine

  North Carolina State University

  2005

• B.S., Animal Science

  North Carolina State University

  2003