JoAnne Stubbe

About

JoAnne Stubbe is a professor in the Department of Chemistry and Chemical Biology at Harvard University. She studies ribonucleotide reductases (RNRs), which catalyze the conversion of nucleotides to deoxynucleotides and are essential for DNA replication and repair. Her research supports a range of ongoing projects, including those related to the radical propagation pathway utilized by Class I RNRs, the interactions between protein subunits of Class I RNR, the regulation of RNRs, and the mechanisms behind clinical drugs. She also examines the biosynthesis, activation, and regulation of the formation of the essential metallo-cofactors of RNRs in organisms such as E. coli, S. cerevisiae, and humans.

Research topics

• Computer Science
• Biochemistry
• Computational biology
• Chemistry
• Biology

Selected publications

• Basis of dATP inhibition of RNRs

  Journal of Biological Chemistry · 2018-06-01 · 6 citations

  articleOpen accessSenior authorCorresponding

  Ribonucleotide reductases (RNRs) are essential enzymes producing de novo deoxynucleotide (dNTP) building blocks for DNA replication and repair and regulating dNTP pools important for fidelity of these processes. A new study reveals that the class Ia Escherichia coli RNR is regulated by dATP via stabilization of an inactive α4β4 quaternary structure, slowing formation of the active α2β2 structure. The results support the importance of the regulatory α4β4 complex providing insight in design of exp…

  PublisherOA PDFDOI

• A Ferredoxin Disulfide Reductase Delivers Electrons to the to the Methanosarcina barkeri Class III Ribonucleotide Reductase

  Applied Categorical Structures · 2015-12-01 · 2 citations

  articleSenior author
  Publisher

• Mono-fluorotryptophans as probes of proton-coupled electron transfer in biology

  Journal of Inorganic Biochemistry · 2025-11-08

  articleOpen access
  PublisherOA PDFDOI

• The road less traveled: For love of detection, discovery, and all things radical in nature

  C&EN Global Enterprise · 2020

  1st authorCorresponding

  Let me start by saying that I am grateful for having been paid to pursue my passion for so many years and more than a little taken aback by being named the Priestley Medal winner this year. While I have delivered hundreds of lectures, giving an after-dinner talk about myself that is entertaining and interesting, for me, a scientist’s scientist, is impossible. My task tonight is to describe a little about myself, how I became a biochemist interested in catalysis, and my lab’s scientific contribut…

  PublisherDOI

• An endogenous dAMP ligand in

  DSpace@MIT (Massachusetts Institute of Technology) · 2018-04-01

  articleOpen accessSenior author

  © 2018 National Academy of Sciences. All rights reserved. The high fidelity of DNA replication and repair is attributable, in part, to the allosteric regulation of ribonucleotide reductases (RNRs) that maintains proper deoxynucleotide pool sizes and ratios in vivo. In class Ia RNRs, ATP (stimulatory) and dATP (inhibitory) regulate activity by binding to the ATP-cone domain at the N terminus of the large α subunit and altering the enzyme’s quaternary structure. Class Ib RNRs, in contrast, have a…

  Publisher

Recent grants

• NIH Grant R01GM032191

  NIH · $3.6M · 2005

• NIH Grant R01GM029595

  NIH · $7.9M · 2019

• NIH Grant R01GM034454

  NIH · $4.9M · 2007

Frequent coauthors

• Marina Bennati

  University of Göttingen

  74 shared

• John W. Kozarich

  The University of Texas at Austin

  52 shared

• John H. Robblee

  Momenta Pharmaceuticals (United States)

  47 shared

• Deborah L. Perlstein

  37 shared

• Robert G. Griffin

  Massachusetts Institute of Technology

  37 shared

• Daniel G. Nocera

  34 shared

• Dana E. Vanderwall

  34 shared

• Mingxia Huang

  Shenyang Jianzhu University

  34 shared

Labs

• Nocera GroupPI

Education

• Ph.D., Chemistry

  Harvard University

  1989

• B.S., Chemistry

  University of California, Berkeley

  1984

Similar researchers at Harvard University

• Xiaowei Zhuangh-158
• Joonho Leeh-134
• Eric Jacobsenh-123