J. Paul Robinson

Flow Cytometry: The Next Revolution

Cells · 2023-07-17 · 168 citations

Unmasking the subtleties of the immune system requires both a comprehensive knowledge base and the ability to interrogate that system with intimate sensitivity. That task, to a considerable extent, has been handled by an iterative expansion in flow cytometry methods, both in technological capability and also in accompanying advances in informatics. As the field of fluorescence-based cytomics matured, it reached a technological barrier at around 30 parameter analyses, which stalled the field unti…

Flow Cytometry: Past and Future

BioTechniques · 2022-04-01 · 112 citations

Flow cytometry is a single-cell technology that measures scatter and fluorescence to establish a set of unique cellular properties. Flow cytometry is used in many areas of science, in particular biotechnology and medicine, but also in industrial applications. Flow cytometry can identify multiple phenotypic subsets from a mixture, select a single cell and even isolate that cell by a process called cell sorting. The field is currently undergoing dramatic changes. We are moving rapidly from the pol…

Detection of E. coli labeled with metal-conjugated antibodies using lateral-flow assay and laser-induced breakdown spectroscopy

Analytical and Bioanalytical Chemistry · 2020-01-27 · 27 citations

Identifying antibiotic-resistant strains via cell sorting and elastic-light-scatter phenotyping

Applied Microbiology and Biotechnology · 2024-07-03 · 10 citations

The proliferation and dissemination of antimicrobial-resistant bacteria is an increasingly global challenge and is attributed mainly to the excessive or improper use of antibiotics. Currently, the gold-standard phenotypic methodology for detecting resistant strains is agar plating, which is a time-consuming process that involves multiple subculturing steps. Genotypic analysis techniques are fast, but they require pure starting samples and cannot differentiate between viable and non-viable organi…

Enhancing Sensitivity of Commercial Gold Nanoparticle-Based Lateral Flow Assays: A Comparative Study of Colorimetric and Photothermal Approaches

Sensors · 2025-08-08 · 3 citations

Lateral flow assays (LFAs) are extensively utilized in point-of-care diagnostics due to their affordability, simplicity, and rapid time-to-results. However, their low sensitivity remains a significant limitation, particularly for detecting foodborne pathogens at concentrations below regulatory thresholds. This study evaluated two distinct sensing modalities—photothermal speckle imaging and colorimetric line intensity analysis—for their potential to enhance the sensitivity of commercially availab…