J. Martin Bollinger

About

J. Martin Bollinger is a Professor of Chemistry and Biochemistry and Molecular Biology at The Pennsylvania State University. His research focuses on the mechanisms of metalloenzymes and metallofactor assembly. He is affiliated with the Molecular, Cellular, and Integrative Biosciences program and is involved in various institutes and centers related to bioremediation, infectious disease, industrial biotechnology, and neuroscience. His work includes studying heme oxygenase-like metalloenzymes, cyanobacterial halogenases, radical-initiating metallocofactors, and iron-dependent oxygenases, contributing to the understanding of enzyme mechanisms and biosynthesis pathways.

Research topics

• Chemistry
• Biochemistry
• Stereochemistry
• Biology
• Internal medicine
• Photochemistry
• Medicinal chemistry
• Organic chemistry
• Virology

Selected publications

• Fe-S cofactors in the SARS-CoV-2 RNA-dependent RNA polymerase are potential antiviral targets

  Science · 2021 · 126 citations

  Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causal agent of COVID-19, uses an RNA-dependent RNA polymerase (RdRp) for the replication of its genome and the transcription of its genes. We found that the catalytic subunit of the RdRp, nsp12, ligates two iron-sulfur metal cofactors in sites that were modeled as zinc centers in the available cryo-electron microscopy structures of the RdRp complex. These metal binding sites are essential for replication and for interaction with…

  DOI

• A Peroxodiiron(III/III) Intermediate Mediating Both <i>N</i> -Hydroxylation Steps in Biosynthesis of the <i>N</i> -Nitrosourea Pharmacophore of Streptozotocin by the Multi-domain Metalloenzyme SznF

  Journal of the American Chemical Society · 2020 · 81 citations

  xidases and oxygenases (HDOs).

  DOI

• Heme biosynthesis depends on previously unrecognized acquisition of iron-sulfur cofactors in human amino-levulinic acid dehydratase

  Nature Communications · 2020 · 71 citations

  ] cluster acquisition and coordination. The enzymatic activity of human ALAD is greatly reduced upon loss of its Fe-S cluster, which results in reduced heme biosynthesis in human cells. As ALAD provides an early Fe-S-dependent checkpoint in the heme biosynthetic pathway, our findings help explain why heme biosynthesis depends on intact ISC biogenesis.

  DOI

• An iron–sulfur cluster in the zinc-binding domain of the SARS-CoV-2 helicase modulates its RNA-binding and -unwinding activities

  Proceedings of the National Academy of Sciences · 2023-08-08 · 47 citations

  articleOpen access

  Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, uses an RNA-dependent RNA polymerase along with several accessory factors to replicate its genome and transcribe its genes. Nonstructural protein (nsp) 13 is a helicase required for viral replication. Here, we found that nsp13 ligates iron, in addition to zinc, when purified anoxically. Using inductively coupled plasma mass spectrometry, UV-visible absorption, EPR, and Mössbauer spectroscopies, we char…

  PublisherOA PDFDOI

• An Iron(IV)–Oxo Intermediate Initiating <scp>l</scp>-Arginine Oxidation but Not Ethylene Production by the 2-Oxoglutarate-Dependent Oxygenase, Ethylene-Forming Enzyme

  Journal of the American Chemical Society · 2021 · 47 citations

  ]-l-Arg slows decay of the ferryl complex by >16-fold, implying that RO is initiated by hydrogen-atom transfer (HAT) from C5. That this large substrate deuterium kinetic isotope effect has no impact on the EF:RO partition ratio implies that the same ferryl intermediate cannot be on the EF pathway; the pathways must diverge earlier. Consistent with this conclusion, the variant enzyme bearing the Asp191Glu ligand substitution accumulates ∼4 times as much of the ferryl complex as the wild-type enzy…

  DOI

Recent grants

• NIH Grant R01DK074641

  NIH · $1.1M · 2011

• Mechanisms and Reprogramming of Iron/2-Oxoglutarate Desaturases and Oxacyclases

  NIH · $1.0M · 2016–2021

• NIH Grant R01GM055365

  NIH · $3.7M · 2013

Frequent coauthors

• Carsten Krebs

  Pennsylvania State University

  211 shared

• Boi Hanh Huynh

  64 shared

• Eric W. Barr

  University of Pennsylvania

  59 shared

• Lana Saleh

  55 shared

• Christopher T. Walsh

  Met Office

  52 shared

• Jeffrey Baldwin

  The University of Texas Southwestern Medical Center

  46 shared

• Edward I. Solomon

  SLAC National Accelerator Laboratory

  44 shared

• Brenda A. Ley

  Pennsylvania State University

  43 shared

Similar researchers at Pennsylvania State University

• Sally Assmannh-82
• Philip Bevilacquah-64
• Seth Bordensteinh-64