Nitrous Oxide Consumption in Oxygenated and Anoxic Estuarine Waters

Geophysical Research Letters · 2022 · 21 citations

• Environmental chemistry
• Environmental science
• Chemistry

Abstract Estuaries emit a large but highly uncertain amount of Nitrous oxide (N 2 O) into the atmosphere. To better understand N 2 O cycling processes in estuaries, we provide the first direct observations of N 2 O consumption in the seasonally anoxic Chesapeake Bay, the largest estuary in the United States. N 2 O consumption rates in anoxic waters reached up to 3.3 nmol L −1 d −1 but were generally undetectable in oxygenated waters. However, N 2 O consumption rates were substantially enhanced when the oxygen concentration was experimentally decreased in initially oxygenated samples, indicating the potential of N 2 O consumption in oxygenated environments, for example, surface waters. These potential N 2 O consumption rates followed Michaelis‐Menten kinetics as a function of increasing N 2 O substrate concentration. N 2 O‐consuming microbes that predominantly contained the clade II nitrous oxide reductase gene were detected throughout the water column. These new observations of environmental controls on N 2 O consumption will benefit the modeling of N 2 O cycling and help to constrain the estuarine N 2 O flux.

Nitrous oxide production in the Chesapeake Bay

Limnology and Oceanography · 2022 · 38 citations

• Environmental science
• Environmental chemistry
• Chemistry

Abstract Estuaries at the global scale are significant but highly uncertain sources of atmospheric nitrous oxide (N 2 O), which is an intense greenhouse gas and ozone depletion agent. As the largest estuary in the United States, the Chesapeake Bay is suggested to be a spatially and temporally variable source and sink of N 2 O. However, limited observations of N 2 O cycling preclude us from estimating and predicting its net N 2 O flux. To improve our mechanistic understanding of the processes that control the N 2 O flux at the point of production, we applied multiple 15 N tracers (, 15 N‐urea, and ) to separately track N 2 O production from nitrification and denitrification under in situ and manipulated O 2 concentrations in the Chesapeake Bay. Nitrification was the major N 2 O production pathway in oxic waters (up to 7.5 nmol N 2 O L −1 d −1 ). In contrast, denitrification dominated N 2 O production from hypoxic/anoxic waters (up to 20 nmol N 2 O L −1 d −1 ). N 2 O production from urea was observed for the first time in estuarine waters. The contribution from urea was small, but interestingly, showed a depth pattern distinct from other N 2 O precursors. Experimentally lowering the O 2 concentration substantially enhanced N 2 O production. Therefore, the expansion of hypoxic and anoxic zones in the Chesapeake Bay under climate change as suggested by some climate models may favor the production of N 2 O, potentially providing positive feedback on warming. Overall, our study provides mechanistic constraints on N 2 O dynamics that could benefit modeling studies to better estimate the N 2 O flux in the Chesapeake Bay and other coastal environments.

Microbial N2O consumption in and above marine N2O production hotspots

The ISME Journal · 2020 · 67 citations

• Environmental science
• Environmental chemistry
• Ecology

O budget.