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Dorit Hanein

Dorit Hanein

· Professor of Bioengineering Professor of Chemistry and Biochemistry

University of California, Santa Barbara · Bioengineering

Active 1993–2026

h-index53
Citations10.2k
Papers16847 last 5y
Funding$173.3M1 active

Academic metrics are sourced from OpenAlex and public funding records; values may differ from Google Scholar.

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About

Dorit Hanein is a Professor of Bioengineering and Chemistry and Biochemistry at UC Santa Barbara. Her research focuses on exploring the complexities of macromolecular machines that drive biological processes, including immune responses, sensory perceptions, healing mechanisms, and the processes of maturation and aging. She leads the Hanein Lab, which utilizes advanced cryogenic light and electron microscopes to obtain quantitative representations of biological structures at the atomic scale, allowing for the observation of mechanical alterations within complex three-dimensional environments.

Research topics

  • Cell biology
  • Biochemistry
  • Biology
  • Chemistry
  • Physics
  • Biophysics
  • Medicine

Selected publications

  • Morphological control enables nanometer-scale dissection of cell-cell signaling complexes

    Nature Communications · 2022-12-20 · 11 citations

    articleOpen accessSenior author

    Protein micropatterning enables robust control of cell positioning on electron-microscopy substrates for cryogenic electron tomography (cryo-ET). However, the combination of regulated cell boundaries and the underlying electron-microscopy substrate (EM-grids) provides a poorly understood microenvironment for cell biology. Because substrate stiffness and morphology affect cellular behavior, we devised protocols to characterize the nanometer-scale details of the protein micropatterns on EM-grids b…

    PublisherOA PDFDOI

  • High-resolution yeast actin structures indicate the molecular mechanism of actin filament stiffening by cations

    Communications Chemistry · 2024-07-30 · 6 citations

    articleOpen accessCorresponding

    Actin filament assembly and the regulation of its mechanical properties are fundamental processes essential for eukaryotic cell function. Residue E167 in vertebrate actins forms an inter-subunit salt bridge with residue K61 of the adjacent subunit. Saccharomyces cerevisiae actin filaments are more flexible than vertebrate filaments and have an alanine at this position (A167). Substitution of this alanine for a glutamic acid (A167E) confers Saccharomyces cerevisiae actin filaments with salt-depen…

    PublisherOA PDFDOI

  • Building momentum through networks: Bioimaging across the Americas

    Journal of Microscopy · 2024-05-15 · 5 citations

    articleOpen access

    In September 2023, the two largest bioimaging networks in the Americas, Latin America Bioimaging (LABI) and BioImaging North America (BINA), came together during a 1-week meeting in Mexico. This meeting provided opportunities for participants to interact closely with decision-makers from imaging core facilities across the Americas. The meeting was held in a hybrid format and attended in-person by imaging scientists from across the Americas, including Canada, the United States, Mexico, Colombia,…

    PublisherOA PDFDOI

  • Optimizing Transmembrane Protein Assemblies in Nanodiscs for Structural Studies: A Comprehensive Manual

    BIO-PROTOCOL · 2024-01-01 · 3 citations

    articleOpen accessSenior author

    Membrane protein structures offer a more accurate basis for understanding their functional correlates when derived from full-length proteins in their native lipid environment. Producing such samples has been a primary challenge in the field. Here, we present robust, step-by-step biochemical and biophysical protocols for generating monodisperse assemblies of full-length transmembrane proteins within lipidic environments. These protocols are particularly tailored for cases where the size and molec…

    PublisherDOI

  • Harmonizing the Generation and Pre-publication Stewardship of FAIR Image Data

    PubMed · 2024-01-23 · 2 citations

    preprintOpen access

    . This White Paper details a comprehensive set of requirements for integrated image data and metadata management - from acquisition through dissemination - ensuring the contextual information necessary for assessing quality, interpreting scientific validity, and enabling meaningful reuse remains intrinsically linked to the data throughout its lifecycle. Critically, it recognizes that generating harmonized, well-annotated publicly available corpora of FAIR bioimage data requires these datasets to…

    PublisherOA PDFDOI

Recent grants

Frequent coauthors

  • Niels Volkmann

    239 shared

  • Mark F. Swift

    Scintillon Institute

    82 shared

  • Armel Bezault

    Université Paris Cité

    48 shared

  • Borja Rodríguez de Francisco

    Centro Nacional de Biotecnología

    40 shared

  • Karen Anderson

    39 shared

  • Cécile Sauvanet

    Commissariat à l'Énergie Atomique et aux Énergies Alternatives

    37 shared

  • Xiaoping Xu

    Second People’s Hospital of Yibin

    34 shared

  • Fernando Vilela

    Université Paris Cité

    26 shared

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