About

Timothy J. Kamp is a Professor in Medicine and Co-director of the Stem Cell and Regenerative Medicine Center at the University of Wisconsin–Madison. His research interests focus on cardiogenesis from embryonic stem cells, exploring the processes involved in heart development and regeneration. His work contributes to understanding how stem cells can be directed to form cardiac tissue, which has implications for regenerative medicine and treatment of heart diseases.

Research topics

• Medicine
• Internal medicine
• Genetics
• Biology
• Cardiology
• Bioinformatics
• Cell biology
• Pharmacology
• Endocrinology
• Intensive care medicine
• Pathology

Selected publications

• Early multi-omic signatures and machine learning models predict cardiomyocyte differentiation efficiency and enable robust hPSC differentiation to cardiomyocytes

  bioRxiv (Cold Spring Harbor Laboratory) · 2026-01-09

  articleOpen access

  Protocols for generating cardiomyocytes (CMs) from human pluripotent stem cells (hPSCs) have existed for nearly two decades, yet manufacturing variability in terminal cell identity continues to limit clinical translation. To uncover the origin of fate divergence during hPSC-CM differentiation, we performed temporal transcriptomics, proteomics, and metabolomics of high and low efficiency differentiations. We identified significant early multi-omic divergence between differentiation batches and key pathways underlying fate divergence at critical differentiation stages included Wnt, MAPK, and glucose metabolism. Machine learning models trained on early candidate gene markers predicted hPSC-CM purity better than models using canonical cardiac development markers. Lastly, multi-omic insights informed perturbations, including Wnt and MAPK inhibition, which produced higher CM purities and yields. Our results showcase multi-omic analysis coupled with machine learning models as a powerful tool to identify cell fate determinants and enable robust manufacturing of complex cell products such as hPSC-derived cell therapies.

  PublisherOA PDFDOI

• PO-04-219 CARDIAC TISSUE ON A CHIP, A NEW HIPSC-CM MODELING APPROACH TO BRUGADA SYNDROME

  Heart Rhythm · 2026-04-01

  articleSenior author
  PublisherDOI

• Caveolar Compartmentalization of Pacemaker Signaling Ensures Stable Sinoatrial Rhythmicity Which Is Disrupted in Heart Failure

  JACC. Clinical electrophysiology · 2026-02-01 · 1 citations

  articleOpen access

  BACKGROUND: Caveolae are nanoscale, plasma membrane invaginations that compartmentalize ion channels and transporters, including those involved in sinoatrial node (SAN) activity. However, role of caveolae in cardiac pacemaking remains unknown. OBJECTIVES: This study sought to determine the role of caveolae in SAN pacemaking and sinus node dysfunction (SND). METHODS: imaging, immunofluorescent and electron microscopy were performed in wild-type, cardiac-specific Cav3 knockout and 8-week post-myocardial infarction heart failure mice. Mouse and human donor SAN tissues were used for biochemical protein copurification studies. A novel 3-dimensional single SAN cell mathematical model was used to determine the impact of protein localization on SAN pacemaking. RESULTS: release event uncoupling and SND. CONCLUSIONS: SAN pacemaking is driven by complex protein interactions within a nanoscale caveolar pacemaker signalosome. Disruption of caveolae leads to SND, demonstrating a new dimension of SAN remodeling and revealing a novel therapeutic target.

  PublisherDOI

• PO-04-192 LMNA MUTATION DRIVES ELECTRICAL REMODELING AND ARRHYTHMOGENICITY IN HUMAN CARDIAC MICROTISSUES

  Heart Rhythm · 2026-04-01

  article
  PublisherDOI

• Derivation of Chamber-specific Cardiomyocytes from Human iPSCs Defined by Single Cell Transcriptomics for Disease Modeling and Therapy

  Journal of Molecular and Cellular Cardiology Plus · 2026-03-01

  articleOpen accessSenior author
  PublisherDOI

• PO-04-193 A PREDICTIVE HIPSC-BASED EXPERIMENTAL AND COMPUTATIONAL FRAMEWORK FOR MECHANISTIC PHARMACOLOGY AND IDIOPATHIC VENTRICULAR FIBRILLATION DISEASE MODELING

  Heart Rhythm · 2026-04-01

  article
  PublisherDOI

• BPS2026 - Regulation of L-type calcium channels and calcium cycling by LRRC10 in hiPSC-cardiomyocytes

  Biophysical Journal · 2026-02-01

  articleSenior author
  PublisherDOI

• Gut microbiota modulation in cardiac cell therapy with immunosuppression in a nonhuman primate ischemia/reperfusion model

  npj Regenerative Medicine · 2025-01-15 · 5 citations

  articleOpen access

  Gut microbiota affect transplantation outcomes; however, the influence of immunosuppression and cell therapy on the gut microbiota in cardiovascular care remains unexplored. We investigated gut microbiota dynamics in a nonhuman primate (NHP) cardiac ischemia/reperfusion model while under immunosuppression and receiving cell therapy with human induced pluripotent stem cell (hiPSC)-derived endothelial cells (EC) and cardiomyocytes (CM). Both immunosuppression and EC/CM co-treatment increased gut microbiota alpha diversity. Immunosuppression promoted anaerobes, such as Faecalibacterium, Streptococcus, Anaerovibrio and Dialister, and altered amino acid metabolism and nucleosides/nucleotides biosynthesis in host plasma. EC + CM cotreatment favors Phascolarctobacterium, Fusicatenibacter, Erysipelotrichaceae UCG-006, Veillonella and Mailhella. Remarkably, gut microbiota of the EC/CM co-treatment group resembled that of the pre-injury group, and the NHPs exhibited a metabolic shift towards amino acid and fatty acid/lipid biosynthesis in plasma following cell therapy. The interplay between shift in microbial community and host homeostasis during treatment suggests gut microbiome modulation could improve cell therapy outcomes.

  PublisherOA PDFDOI

• BPS2025 - LRRC10 exerts L-type Ca2+ current-specific modulation through interaction with the N-terminus of CaV1.2

  Biophysical Journal · 2025-02-01

  articleSenior author
  PublisherDOI

• Stem cell-derived heterocellular atrial engineered cardiac tissue with comparisons to native human atrial myocardium

  American Journal of Physiology-Heart and Circulatory Physiology · 2025-10-07

  articleOpen access

  We demonstrate an atrial-like engineered cardiac tissue that recapitulates adult human atrial contraction for both kinetics and force production. Furthermore, we include numerous previously unmeasured ultrastructure metrics such as sarcomere alignment, cardiomyocyte anisotropy, and caveolae abundance. Our constructs may provide a human cardiac tissue platform for drug testing to identify combinatorial therapies to address atrial contractile dysfunction and diseases linked to cardiomyocyte ultrastructural defects.

  PublisherDOI

Recent grants

• NIH Grant P51RR000167

  NIH · $61.8M · 2013

• NIH Grant R21HL072089

  NIH · $652k · 2006

• NIH Grant R29HL059429

  NIH · $221k · 2001

• Caveolae, T-type Calcium Channels and Cardiac Hypertrophy

  NIH · $1.9M · 2012–2016

• Refining Cardiac Progenitor Cells for Myocardial Repair

  NIH · $1.5M · 2015–2019

Frequent coauthors

• James A. Thomson

  Morgridge Institute for Research

  106 shared

• Jianhua Zhang

  74 shared

• Amanda M. Herman

  Chapman University

  71 shared

• José Jalife

  Spanish National Centre for Cardiovascular Research

  67 shared

• Todd J. Herron

  University of Michigan–Ann Arbor

  66 shared

• Sean P. Palecek

  University of Wisconsin–Madison

  65 shared

• Matthew Klos

  Case Western Reserve University

  61 shared

• Luqia Hou

  Merck & Co., Inc., Rahway, NJ, USA (United States)

  61 shared

Education

• Ph.D., Cardiogenesis

  University of Wisconsin-Madison

  1995

• M.S., Cardiogenesis

  University of Wisconsin-Madison

  1991

• B.S., Biology

  University of Wisconsin-Madison

  1988