Daniel T. Chiu

About

Daniel T. Chiu is the A. Bruce Montgomery Professor of Chemistry and the Endowed Professor in Analytical Chemistry at the University of Washington. His research focuses on analytical chemistry, with particular emphasis on developing innovative methods and techniques for chemical analysis. As a distinguished faculty member, he contributes to advancing the understanding and application of analytical methods in chemistry.

Research topics

• Biochemistry
• Cell biology
• Cancer research
• Chemistry
• Photochemistry
• Materials science
• Genetics
• Biology
• Organic chemistry

Selected publications

• Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots

  Angewandte Chemie International Edition · 2021 · 70 citations

  Senior authorCorresponding

  Reduced nicotinamide adenine dinucleotide (NADH) is a key coenzyme in living cells due to its role as an electron carrier in redox reactions, and its concentration is an important indicator of cell metabolic state. Abnormal NADH levels are associated with age-related metabolic diseases and neurodegenerative disorders, creating a demand for a simple, rapid analytical method for point-of-care NADH sensing. Here we develop a series of NADH-sensitive semiconducting polymer dots (Pdots) as nanoprobes…

  DOI

• Extracellular Vesicles for Clinical Diagnostics: From Bulk Measurements to Single-Vesicle Analysis

  ACS Nano · 2025-07-28 · 51 citations

  reviewOpen access

  Extracellular vesicles (EVs) play a crucial role in intercellular communication, signaling pathways, and disease pathogenesis by transporting biomolecules such as DNA, RNA, proteins, and lipids derived from their cells of origin, and they have demonstrated substantial potential in clinical applications. Their clinical significance underscores the need for sensitive methods to fully harness their diagnostic potential. In this comprehensive review, we explore EV heterogeneity related to biogenesis…

  PublisherDOI

• Comparison of EV characterization by commercial high‐sensitivity flow cytometers and a custom single‐molecule flow cytometer

  Journal of Extracellular Vesicles · 2024-08-01 · 39 citations

  articleOpen accessSenior authorCorresponding

  Abstract High‐sensitivity flow cytometers have been developed for multi‐parameter characterization of single extracellular vesicles (EVs), but performance varies among instruments and calibration methods. Here we compare the characterization of identical (split) EV samples derived from human colorectal cancer (DiFi) cells by three high‐sensitivity flow cytometers, two commercial instruments, CytoFLEX/CellStream, and a custom single‐molecule flow cytometer (SMFC). DiFi EVs were stained with the m…

  PublisherOA PDFDOI

• Phase 2 of extracellular RNA communication consortium charts next-generation approaches for extracellular RNA research

  iScience · 2022-06-23 · 30 citations

  reviewOpen access

  The extracellular RNA communication consortium (ERCC) is an NIH-funded program aiming to promote the development of new technologies, resources, and knowledge about exRNAs and their carriers. After Phase 1 (2013-2018), Phase 2 of the program (ERCC2, 2019-2023) aims to fill critical gaps in knowledge and technology to enable rigorous and reproducible methods for separation and characterization of both bulk populations of exRNA carriers and single EVs. ERCC2 investigators are also developing new b…

  PublisherOA PDFDOI

• Ultrabright Pdots with a Large Absorbance Cross Section and High Quantum Yield

  ACS Applied Materials & Interfaces · 2022-03-08 · 21 citations

  articleOpen accessSenior authorCorresponding

  Semiconducting polymer dots (Pdots) are increasingly used in biomedical applications due to their extreme single-particle brightness, which results from their large absorption cross section (σ). However, the quantum yield (Φ) of Pdots is typically below 40% due to aggregation-induced self-quenching. One approach to reducing self-quenching is to use FRET between the donor (D) and acceptor (A) groups within a Pdot; however, Φ values of FRET-based Pdots remain low. Here, we demonstrate an approach…

  PublisherOA PDFDOI

Recent grants

• High-precision mapping of the spatial organization of synaptic-vesicle membrane proteins

  NIH · $1.9M · 2017–2023

• Digital LAMP as a point of care assay for HPV detection

  NIH · $1.9M · 2016–2020

• NIH Grant R21GM103459

  NIH · $378k · 2015

Frequent coauthors

• Mei‐Ling Cheng

  Chang Gung Memorial Hospital

  105 shared

• Jiangbo Yu

  79 shared

• Changfeng Wu

  78 shared

• Hung‐Yao Ho

  Chang Gung Memorial Hospital

  73 shared

• Owe Orwar

  44 shared

• Bryant S. Fujimoto

  University of Washington

  41 shared

• Fangmao Ye

  University of Washington

  41 shared

• Perry G. Schiro

  38 shared

Education

• B.A., Neurobiology

  University of California at Berkeley

  1993

• B.S., Chemistry

  University of California at Berkeley

  1993

• Ph.D., Chemistry

  Stanford University

  1998

Awards & honors

• American Chemical Society National Fresenius Award
• Pittcon Achievement Award
• Fellow of the AAAS (2011)
• Keck Distinguished Young Scholar in Biomedical Research
• Alfred P. Sloan fellow

Similar researchers at University of Washington

• Michael H. Gelbh-111
• Samson A. Jenekheh-108
• Daniel R. Gamelinh-92