About

Brett E Arenz is an Associate Professor in the Department of Plant Pathology at the University of Minnesota. His research focuses on plant diseases, including the epidemiology and management of various plant pathogens. He has contributed to understanding diseases such as tar spot of corn, aster yellows in garlic, and infections caused by viruses like Tobacco Rattle Virus. His work involves developing diagnostic methods, studying pathogen biology, and exploring management practices to control plant diseases. Dr. Arenz's research supports sustainable agriculture and contributes to the broader goals of food security and environmental health.

Research topics

• Biology
• Computer Science
• Agronomy
• Botany
• Library science
• Virology
• Biotechnology
• Pathology
• Medicine
• Horticulture

Selected publications

• First Report of Tobacco Rattle Virus Infecting <i>Weigela florida</i> in the United States

  Plant Disease · 2023

  • Biology
  • Botany
  • Horticulture

  Weigela (Weigela florida (Bunge) A. DC., Family: Caprifoliaceae) are woody shrubs native to North China, Korea, and Japan. In the U.S., weigela are commonly used as landscape ornamental plants (McNamara et al. 2010). Two viruses have been reported in weigela: tomato spotted wilt orthotospovirus and impatiens necrotic spot orthotospovirus (Sastry et al. 2019). Ten weigela plants, originating from commercial nurseries in Minnesota, exhibiting chlorosis, chlorotic line patterns, and necrosis (e-Xtra) were submitted for virus diagnostics as potted plants at the University of Minnesota Plant Disease Clinic and the Virology Lab in 2019 and 2020 (five plants each year). Under greenhouse conditions, symptoms progressed from chlorosis to necrosis and even plant death in two of the five plants in 2019. Electron microscopy revealed rod-shaped particles of ≈20 nm in diameter and lengths between 40-200 nm with similar morphology to members of the genus Tobravirus (e-Xtra). Virus-like particles were enriched by ultracentrifugation and total nucleic acids were extracted from partial purifications using a phenol:chloroform extraction method (Lockhart et al. 1997). Tobacco rattle virus (TRV) was identified by cloning and sequencing of the 463bp amplicon obtained with the TRV detection primers described in Robinson, 1992. High-throughput sequencing (HTS) was done to confirm the TRV detection. A cDNA library was prepared from purified viral RNA using the TruSeq Stranded mRNA kit and sequenced on Illumina NovaSeq 6000 platform as 150 bp-paired end reads. A total of 44,316,446 raw data reads were obtained, preprocessed using the BBDuk plugin, and de novo assembled using SPAdes assembler. Viral contigs were identified using the NCBI BLASTX tool. The assembly of TRV RNA1 was 6,842 nt with 20,627,348 reads (47% of total reads) mapped to it and an average coverage per nucleotide at 323,639X. The assembly of TRV RNA2 was 3,033 nt with 22,769,253 reads (52% of total reads) mapped to it and an average coverage per nucleotide at 798,660X. NCBI GenBank accession numbers for the assemblies representing RNA1 and RNA2 are OQ408335 and OQ408336, respectively. NCBI BLASTn analysis showed the highest level of nucleotide identity to TRV genomic RNA segments 1 and 2, with 97% and 99% identity to the TRV isolate RNA1 (GQ903771) and RNA2 (GQ903772), respectively, that originated from Michigan potato. No other viral contigs were detected from the virion nucleic acid extraction by HTS, however this enrichment method doesn't exclude other viruses. In addition to using the detection primers by Robinson 1992, we designed primers based on our HTS data: TRV-WG-DetF3 5'- GACGAAGGAGGCTGTCATTGC-3' and TRV-WG-DetR3 5'-CGGACTATCGTGATGCCCATGC- 3'. RT-PCR amplicons from each of the 10 symptomatic plants were cloned and sequenced. Among these clones, Sanger sequence identities ranged between 96-100% compared to the HTS data and 98-99% to the TRV potato isolate from MI. To our knowledge, this is the first report of TRV infecting the ornamental host W. florida worldwide. TRV is a nematode-transmitted viral pathogen of economic importance, most notably in potatoes (Sastry et al. 2019). In the US, TRV has been reported on several landscape ornamentals, horticultural crops, and native habitats. Further research is needed to investigate the impact of TRV on the ornamental industry and the role of ornamentals as reservoirs for cultivated crops like potatoes.

  DOI

• Tar Spot of Corn: A Diagnostic and Methods Guide

  Plant Health Progress · 2022 · 16 citations

  • Biology
  • Biotechnology
  • Agronomy

  Tar spot of corn is an emerging plant disease in the continental United States and Canada caused by the fungal pathogen Phyllachora maydis Maubl. Tar spot has been known to occur in Mexico, the Caribbean, and Central and South America since the early to mid-1900s. In 2015, it was reported for the first time in the continental United States. Since that time, tar spot has spread across corn-producing areas in the United States with epidemics as recent as 2021 resulting in significant yield losses. Although tar spot has been known to affect corn for over a century in the Americas, the biology of the pathogen, etiology, and epidemiology of the disease are not well understood. Additionally, symptoms and signs of tar spot resemble other foliar diseases and abiotic disorders of corn, which may lead to misdiagnosis. In this paper, we provide a brief description of current knowledge about tar spot of corn, including pathogen taxonomy, host range, symptoms and signs, specimen storage, pathogenicity testing, diagnostic protocols, and geographic distribution. This information will be useful to diagnosticians, researchers, and other professionals working with this disease.

  PublisherOA PDFDOI

• First Report of Tar Spot of Corn Caused by <i>Phyllachora maydis</i> in Minnesota

  Plant Disease · 2020 · 15 citations

  Senior authorCorresponding
  • Computer Science
  • Library science
  • Biology
  DOI

Frequent coauthors

• W. Schröpp

  Munich University of Applied Sciences

  37 shared

• Robert A. Blanchette

  12 shared

• Benjamin W. Held

  University of Minnesota

  7 shared

• Roberta L. Farrell

  University of Waikato

  7 shared

• Shona M. Duncan

  University of Wisconsin–Stevens Point

  6 shared

• Joel A. Jurgens

  University of Minnesota

  5 shared

• Laura Sweets

  4 shared

• Gerald L. Miller

  Purdue University West Lafayette

  4 shared

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